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Human catestatin ELISA Kit

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人儿茶酚抑素酶联免疫试剂盒（CSB-E17355h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的catestatin含量。人儿茶酚抑素（CST）是一种心血管活性多肽，其与慢性心力衰竭和室性心律失常存在密切联系。研究表明，CST通过调节交感神经活性、抑制炎症反应和促进细胞凋亡等机制，在心血管系统中发挥保护作用，并可能成为治疗相关疾病的新靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品可为神经内分泌调控机制、心血管疾病模型构建、代谢综合征相关研究提供可靠的实验工具。科研人员可通过探究不同生理或病理条件下（如高血压、肥胖、糖尿病等）样本中CST的浓度动态变化，适用于细胞实验、动物模型及临床前研究的生物标志物分析，但不用于临床诊断。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

catestatin
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human catestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	97-105
1:2	Average %	83
	Range %	80-87
1:4	Average %	92
	Range %	87-94
1:8	Average %	91
	Range %	87-95

回收率：

The recovery of human catestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	94	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.564	2.438	2.501	2.317
10	2.004	2.131	2.068	1.884
5	1.533	1.568	1.551	1.367
2.5	0.905	0.935	0.920	0.736
1.25	0.618	0.608	0.613	0.429
0.625	0.432	0.445	0.439	0.255
0.312	0.297	0.287	0.292	0.108
0	0.183	0.185	0.184

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human catestatin antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled catestatin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.