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中文名称:人糖缺失性转铁蛋白(CDT)酶联免疫试剂盒
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货号:CSB-E09602h
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规格:96T/48T
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价格:¥3200/¥2500
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其他:
产品详情
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产品描述:人糖缺失性转铁蛋白(CDT)酶联免疫试剂盒(CSB-E09602h)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CDT含量。试剂盒检测范围为50 ng/mL-1600 ng/mL,适用于科研场景中评估酒精摄入对转铁蛋白糖基化修饰的影响,探究慢性酒精暴露导致的肝脏代谢异常机制,或作为酒精相关疾病动物模型的研究工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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别名:N/A
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缩写:CDT
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:50 ng/mL-1600 ng/mL
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灵敏度:25 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cell Biology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 93-105 1:2 Average % 86 Range % 82-94 1:4 Average % 96 Range % 92-100 1:8 Average % 88 Range % 82-96 -
回收率:
The recovery of human CDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-102 EDTA plasma (n=4) 87 82-93 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 1600 1.849 1.951 1.900 1.865 800 0.983 0.999 0.991 0.956 350 0.476 0.457 0.467 0.432 150 0.264 0.247 0.256 0.221 50 0.104 0.108 0.106 0.071 0 0.034 0.036 0.035 -
数据处理:
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货期:3-5 working days
引用文献
- Does ethyl glucuronide in hair correlates with alcohol consumption? A comparative study with other traditional biomarkers among individuals with alcohol dependence syndrome S Ghosh,Alcohol,2022
- Use of filter paper to measure alcohol biomarkers among opioid-dependent patients on agonist maintenance treatment: A community-based study Quraishi R, et al,Indian Journal of Psychological Medicine,2019
- Carbohydrate Deficient Transferrin and Interleukin-6 as Predictors of Fibrosis in Alcohol Cirrhosis Hanumanthappa Nandeesha. et al,Indian Journal of Clinical Biochemistry,2015
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