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  4. Human calcitonin gene related peptide,CGRP ELISA Kit

Human calcitonin gene related peptide,CGRP ELISA Kit

文献引用 (28) 说明书下载
  • 中文名称：
    人降钙素基因相关肽(CGRP)酶联免疫试剂盒
  • 货号：
    CSB-E08210h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人降钙素基因相关肽（CGRP）酶联免疫检测试剂盒（货号：CSB-E08210h），采用双抗体夹心法定量检测血清、血浆、细胞培养上清液及组织匀浆样本中的CGRP水平，检测灵敏度为1.56-100 pg/mL。CGRP是一种广泛分布于中枢和外周神经系统的神经肽，参与调节血管舒张、痛觉信号传递及炎症反应，尤其在偏头痛和心血管疾病研究中具有重要价值。本试剂盒通过特异性捕获和检测抗体实现高灵敏度定量分析，适用于体外研究CGRP在神经生物学、心血管功能、疼痛机制或炎症模型中的动态变化，例如评估实验动物模型中CGRP的释放水平、细胞因子刺激下培养细胞的分泌特征，或药物干预对组织内CGRP表达的影响等科研用途。试剂盒操作流程标准化，提供预包被板条和完整试剂组分，支持科研人员高效完成样本检测与数据分析。
  • 别名：
    Beta type CGRP ELISA Kit; Beta-CGRP ELISA Kit; Beta-type CGRP ELISA Kit; CALC2 ELISA Kit; Calcb ELISA Kit; CALCB_HUMAN ELISA Kit; Calcitonin gene related peptide 2 ELISA Kit; Calcitonin gene related peptide II ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; Calcitonin related polypeptide; beta ELISA Kit; CGRP II ELISA Kit; CGRP-II ELISA Kit; CGRP2 ELISA Kit; CGRPII ELISA Kit; FLJ30166 ELISA Kit
  • 缩写：
    CGRP
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-92
    1:2Average %96
    Range %91-105
    1:4Average %97
    Range %92-110
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of human CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.660 2.511 2.586 2.432
    502.080 2.152 2.116 1.962
    251.451 1.366 1.409 1.255
    12.50.795 0.744 0.770 0.616
    6.250.491 0.523 0.507 0.353
    3.120.315 0.325 0.320 0.166
    1.560.248 0.244 0.246 0.092
    00.157 0.151 0.154 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

关于人降钙素基因相关肽CGRP ELISA试剂盒（CSB-E08210h），这个CUSABIO的CGRP Sandwich ELISA检测的具体是什么？全长还是片段？这个试验能否检测其他CGRP相关肽？

A:
CSB-E08210h 检测的是Beta-type CGRP，参考https://www.uniprot.org/uniprot/P10092针对全长设计，至于客户提到的other CGRP related peptides理论是检测不了的。如果您有具体信息，可以发送给我们进一步尝试。
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靶点详情

  • 功能：
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 基因功能参考文献：
    1. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
    2. Keratinocyte-derived CGRPbeta may modulate epidermal homeostasis through autocrine/paracrine signaling and may contribute to chronic pain under pathological conditions. PMID: 21641113
    3. Calcitonin gene-related peptide stimulates proliferation of alveolar epithelial cells. PMID: 19192276
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calcitonin family
  • 数据库链接：

    HGNC: 1438

    OMIM: 114160

    KEGG: hsa:797

    STRING: 9606.ENSP00000346017

    UniGene: Hs.534305

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |