Human blca-4 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人BLCA-4酶联免疫检测试剂盒（货号：CSB-E14959h），采用双抗体夹心法定量检测血清、血浆、尿液及组织匀浆样本中膀胱癌特异性生物标志物BLCA-4的水平。BLCA-4是一种与膀胱癌发生密切相关的核基质蛋白，其异常表达可作为膀胱癌早期研究的重要分子靶点。试剂盒检测灵敏度为0.625 ng/mL，线性范围覆盖0.625-40 ng/mL，预包被高特异性抗体可确保检测的特异性，实验过程包含标准品孵育、样本反应、显色及终止步骤，总耗时约4小时。适用于科研领域中对膀胱癌发病机制、肿瘤微环境调控及生物标志物筛查的研究，支持多种生物样本类型的平行检测，为体外实验提供稳定可靠的定量分析工具。试剂盒包含预装微孔板、标准品、检测抗体及全套实验试剂，操作便捷且重复性良好。
缩写：

BLCA-4
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-91
1:2	Average %	99
	Range %	96-103
1:4	Average %	104
	Range %	101-107
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-90
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.