Human apelin 36(AP36)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人apelin 36(AP36)酶联免疫试剂盒（CSB-E13566h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AP36含量。AP36是一种在多囊卵巢综合征（PCOS）患者中发现的肽，其水平与BMI呈正相关，可能与PCOS的发病机制无直接关系，但可能作为受BMI影响的脂肪因子参与其中。研究显示，肥胖PCOS患者的AP36水平高于非肥胖PCOS患者，提示其在肥胖相关PCOS中的作用。试剂盒检测范围为22.86 pg/mL-400 pg/mL，该产品可稳定检测复杂生物样本中的低丰度AP36，为心血管疾病机制研究、代谢调控通路分析、肥胖或糖尿病模型构建等科研项目提供可靠工具，尤其适用于动态监测疾病模型中AP36的分泌变化或药物干预后的生物标志物响应。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

AP36
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

22.86 pg/mL-400 pg/mL
灵敏度：

14.29 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AP36 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	89-106
1:2	Average %	95
	Range %	92-99
1:4	Average %	88
	Range %	85-92
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of human AP36 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	93	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.