Human apelin 12,AP12 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人apelin 12(AP12)酶联免疫试剂盒（CSB-E08639h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、唾液、尿液、组织匀浆样本中的AP12含量。人apelin 12（AP12）是一种多肽，对孤儿受体APJ具有高亲和力，参与体液平衡调节和摄食中枢控制，阻断HIV-1通过APJ受体进入，并具有神经保护作用。研究显示，AP12在心血管疾病、神经系统疾病等方面具有潜在应用价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，特别适用于基础医学研究中AP12在代谢调控机制探索、心血管疾病模型建立及生物标志物筛选等方向的应用，为体外检测不同生理或病理条件下AP12在体液或组织中的动态表达提供可靠工具，满足科研人员对多类型样本的并行分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

AP12
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, saliva, urine, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AP12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-95
1:2	Average %	87
	Range %	84-90
1:4	Average %	84
	Range %	81-87
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of human AP12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	89	85-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.