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Human anti glial fibrillary acidic protein (GFAP) autoantibody ELISA kit

  • 中文名称:
    人抗神经胶质纤维酸性蛋白(GFAP)自身抗体酶联免疫试剂盒
  • 货号:
    CSB-EQ027671HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO试剂盒采用间接酶联免疫吸附法,专为定性检测人血清或血浆样本中抗神经胶质纤维酸性蛋白(GFAP)自身抗体而设计。GFAP是星形胶质细胞特异性中间丝蛋白,在中枢神经系统损伤、神经退行性疾病及神经炎症反应中发挥重要调控作用,其自身抗体的存在与多发性硬化、脑炎等神经系统疾病的研究密切相关。实验通过将重组GFAP抗原预包被于微孔板,样本中特异性抗体与固相抗原结合后,再经酶标记二抗催化底物显色,实现目标抗体的定性分析。试剂盒包含预包被板、校准品、酶结合物及显色系统等核心组分,建议储存于-20℃且有效期为6个月,检测浓度范围需根据实验需求另行确认。本产品适用于神经科学基础研究,可用于探索神经损伤标志物、评估疾病模型动物体液免疫应答,或为神经系统自身免疫疾病的机制研究提供实验依据,严格限于科研用途。
  • 缩写:
    GFAP auto-Ab
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    Request Information
  • 灵敏度:
    Request Information
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    qualitative
  • 测定方法:
    Indirect
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 标准曲线:

    Test parameter

    specification

    test result

    Positive control

    >0.8

    2.350

    Negative control

    <0.2

    0.118

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 货期:
    3-5 working days

产品评价

相关问答

 常见问题解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
The customer also needs to know which isotype can be detected by the kit?
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available),expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry!
CSB-EQ027671HU
This kit is for qualitative determination of GFAP autoantibody in human serum and plasma.
There is no capture antibody and detection antibody. There is no standard either in it.
The plate is coated with specific antigen.
Q:

If there is no detection antibody in the kit please can you clarify how the signal is created in the color reaction. Is there labeled antigen rather than a detection antibody?
The principle seems to mention the incorporation of anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). If this is correct then which types of Ig are detected, is it all of them or just certain such as IgG and IgM.

A:
CSB-EQ027671HU
This is an indirect kit. There is no detection antibody in the kit. The target is GFAP autoantibody(it is total, including IgG/IgM). The plate is coated with specific antigen( the antigen binds to the target specifically). The secondary antibody in the kit is anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP)(the secondary antibody binds to the target). Then add TMB substrate, and the color will be developed under the catalysis of HRP.
Q:

The customer specifically asked what isotypes this kit would detect. The answer to their question lies in the secondary antibody being used in the assay. Most commercial antibodies against Immunoglobulins will be specific to a certain isotype IgG, IgM, IgA etc.
I have tried to find examples of commercially available antibodies which would recognize all immunoglobulins however so far I have not been able to find any. Is the secondary antibody you use a mixture of antibodies that will bind to several different isotypes? If so do you know which ones?

A:
The secondary antibody is anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP).
It is mixed. It detects total GFAP autoantibody(including IgG/IgM).
Q:

Regarding your ELISA kit CSB-EQ027671HU, could you please advise what isotypes this kit is capable of detecting? All isotypes, or specific ones?

A:
Thanks for your inquiry!
CSB-EQ027671HU
This kit detects total antibodies, including all isotypes.