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中文名称:人核仁纤维蛋白抗体(AFA/snoRNP/U3RNP)酶联免疫试剂盒
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货号:CSB-E09697h
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规格:96T/48T
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价格:¥3200/¥2500
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其他:
产品详情
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产品描述:CUSABIO人核仁纤维蛋白抗体(AFA/snoRNP/U3RNP)酶联免疫检测试剂盒(货号CSB-E09697h),采用双抗体夹心法原理,适用于定量检测血清样本中靶标抗体的表达水平。核仁纤维蛋白抗体属于一类与核仁结构相关的自身抗体,其异常表达与系统性硬化症、重叠综合征等自身免疫性疾病相关,在科研中常用于探索疾病发生机制及生物标志物研究。本试剂盒操作需使用100μL血清样本,通过两次室温孵育(每次60分钟)完成抗原抗体结合反应,最终通过比色法在450nm波长下测定吸光度值。试剂盒包含预包被微孔板、检测抗体、标准品、HRP酶结合物及显色底物等核心组分,所有组分需在-20°C保存且有效期为6个月。该产品可为科研人员提供稳定可靠的数据支持,用于体外实验研究抗体水平与疾病进程的关联性、药物干预效果评估或相关分子通路机制解析,但不适用于临床诊断及其他非科研用途。具体检测范围及标准曲线信息需联系技术支持获取。
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缩写:AFA/snoRNP/U3RNP
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种属:Homo sapiens (Human)
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样本类型:serum
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检测范围:Request Information
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灵敏度:Request Information
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Epigenetics and Nuclear Signaling
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
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标准曲线:
Test parameter
specification
test result
Positive control
>1.0
1.616
Negative control
<0.1
0.057
Positive rate
10,Positive
100%
Negative rate
10,Negative
100%
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货期:3-5 working days
引用文献
- Impact of Plasminogen Activator Inhibitor-1 Serum Levels and the-675 4G/5G Variant in the SERPINE1 Gene on Systemic Sclerosis in a Mexican Population JA Lomelí-Nieto, JF Muñoz-Valle, JE Navarro-Zarza,Life,2024
- Transforming Growth Factor Beta Isoforms and TGF-βR1 and TGF-βR2 Expression in Systemic Sclerosis Patients JA Lomelí-Nieto,Researchsquare,2022
- Macrophage migration inhibitory factor polymorphisms are a potential susceptibility marker in systemic sclerosis from southern Mexican population: association with MIF mRNA expression and cytokine profile Banos-Hernandez CJ, et al,Clinical Rheumatology,2019
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