Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit

• A 96-well Assay plate --The 96-well plate has been pre-coated with CCP antigen.
• Negative Control (1 x 0.8 ml)
• Positive Control (1 x 0.8 ml)
• HRP-conjugated IgG antibody(1 x 10m)l --Bind to the human anti-CCP antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
• Sample Diluent (2 x 20 ml) --Dilute the sample to an appropriate concentration.
• Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
• Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
• Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
• Stop Solution (1 x 5ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells)
• An Instruction manual

显示更多

收起更多

• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

显示更多

收起更多