Human anti-Survivin(Surv) antibody ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心法定量检测人血清、血浆中存活素（Survivin）蛋白的含量，检测范围为0.625-40 ng/mL，适用于探索该靶点在细胞凋亡调控与肿瘤发生机制中的科研应用。Survivin作为凋亡抑制蛋白家族成员，在多种恶性肿瘤中特异性高表达，参与细胞周期调控与化疗耐药性研究，是肿瘤标志物筛选和抗肿瘤药物开发的重要研究对象。该试剂盒通过预包被特异性捕获抗体和标记检测抗体的协同作用，结合显色反应实现精准定量，标准操作流程可在3小时内完成检测。科研人员可将其用于体外培养细胞上清液、实验动物模型血清或临床前研究血浆样本中Survivin蛋白表达水平的动态监测，为肿瘤发生机制解析、药物敏感性评估及治疗方案优化提供数据支持，实验全程需配合酶标仪进行OD值读取分析。
缩写：

Surv Ab
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-90
1:2	Average %	96
	Range %	90-105
1:4	Average %	86
	Range %	81-90
1:8	Average %	93
	Range %	85-100

回收率：

The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	87	82-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.