Human anti-Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) antibody ELISA Kit
-
中文名称:人粒细胞巨噬细胞集落刺激因子(GM-CSF)抗体(酶联免疫试剂盒
-
货号:CSB-E11438h
-
规格:96T/48T
-
价格:¥3200/¥2500
-
其他:
产品详情
-
产品描述:CUSABIO人粒细胞巨噬细胞集落刺激因子(GM-CSF)抗体检测的定量分析试剂盒(货号CSB-E11438h),基于双抗体夹心法原理设计,适用于血清样本中GM-CSF蛋白的精准检测。GM-CSF作为一种多功能细胞因子,在免疫调节、炎症反应及造血细胞分化中发挥关键作用,其表达水平与炎症性疾病、自身免疫疾病及肿瘤微环境调控密切相关。该试剂盒通过预包被高亲和力抗体实现0.625 ng/mL至40 ng/mL的宽动态检测范围,具备优异的检测灵敏度和批间一致性,实验全程可在3.5小时内完成,包含标准品、检测抗体及显色系统在内的完整组分经严格验证,确保实验稳定性和数据可靠性。科研人员可将其应用于探索GM-CSF在疾病发生发展中的生物学机制、评估药物干预对细胞因子网络的影响,或作为免疫相关动物模型研究的配套检测工具,为免疫学、肿瘤学及炎症性疾病领域的基础研究提供有效的实验支持。
-
缩写:GM-CSF Ab
-
种属:Homo sapiens (Human)
-
样本类型:serum
-
检测范围:0.625 ng/mL-40 ng/mL
-
灵敏度:0.156 ng/mL
-
反应时间:1-5h
-
样本体积:50-100ul
-
检测波长:450 nm
-
研究领域:Immunology
-
测定原理:quantitative
-
测定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 85-101 1:2 Average % 96 Range % 84-100 1:4 Average % 96 Range % 90-103 1:8 Average % 95 Range % 82-106 -
回收率:
The recovery of human GM-CSF antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 86-101 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 2.597 2.648 2.623 2.518 20 2.123 2.131 2.127 2.022 10 1.466 1.472 1.469 1.364 5 0.831 0.896 0.864 0.759 2.5 0.472 0.494 0.483 0.378 1.25 0.312 0.297 0.305 0.200 0.625 0.225 0.241 0.233 0.128 0 0.102 0.107 0.105 -
数据处理:
-
货期:3-5 working days
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-