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Human angiotensin Ⅱ receptor 1 Antibody,ATⅡR1 Ab ELISA Kit

  • 中文名称:
    人血管紧张素Ⅱ受体1抗体(ATⅡR1)酶联免疫试剂盒
  • 货号:
    CSB-E10374h
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

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  • 产品描述:
    人血管紧张素Ⅱ受体1抗体(ATⅡR1)酶联免疫试剂盒(CSB-E10374h)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ATⅡR1 Ab含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL,适用于心血管疾病机制研究、药物干预效果评估或相关动物模型中的靶标分子水平分析,为探索ATⅡR1在体液及组织中的表达规律提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    angiotensin 2 receptor 1 antibody, AT2R1-Ab
  • 缩写:
    ATⅡR1 Ab
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ATⅡR1 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %87-105
    1:2Average %94
    Range %84-100
    1:4Average %98
    Range %92-101
    1:8Average %94
    Range %82-105
  • 回收率:
    The recovery of human ATⅡR1 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-105
    EDTA plasma (n=4)9585-103
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.612 2.662 2.637 2.491
    101.973 1.939 1.956 1.810
    51.294 1.304 1.299 1.153
    2.50.772 0.754 0.763 0.617
    1.250.474 0.498 0.486 0.340
    0.6250.346 0.354 0.350 0.204
    0.3120.255 0.254 0.255 0.109
    00.147 0.145 0.146  
  • 数据处理:
  • 货期:
    3-5 working days

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