Human angiotensin Ⅰ,Ang-Ⅰ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人血管紧张素Ⅰ（Angiotensin I，Ang-Ⅰ）酶联免疫检测试剂盒（货号：CSB-E08527h），基于双抗体夹心法定量原理开发，适用于检测血清、血浆及细胞培养上清液中的Ang-Ⅰ水平，定量检测范围为15.6-1000 pg/mL，灵敏度达15.6 pg/mL。Ang-Ⅰ是肾素-血管紧张素系统（RAS）的关键前体肽，由血管紧张素原经肾素切割生成，进一步转化为Ang-Ⅱ后可介导血管收缩、血压调节及水盐代谢等生理过程，其定量检测对心血管疾病、肾脏功能及高血压机制研究具有重要意义。试剂盒包含预包被特异性抗体的96孔板、标准品及检测所需试剂，支持在3.5小时内完成检测流程，操作便捷且具备良好的样本兼容性。该产品可广泛应用于体外模型研究、药物干预实验及病理生理学分析等领域，实验时需注意避免样本反复冻融或溶血，以确保检测结果的稳定性。本试剂盒仅供科学研究使用，不适用于临床诊断或其他用途。
别名：

angiotensin Ⅰ,Ang-Ⅰ,angiotensin 1,Ang-1
缩写：

Ang-Ⅰ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	98
	Range %	94-103
1:4	Average %	105
	Range %	100-110
1:8	Average %	108
	Range %	102-114

回收率：

The recovery of human Ang-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.