Human alpha-amylase(AMY1) ELISA Kit

amylase; salivary; B ELISA Kit; 1 4 alpha D glucan glucanohydrolase ELISA Kit; 1 ELISA Kit; 1,4 alpha D glucan glucanohydrolase 2B ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; 4-alpha-D-glucan glucanohydrolase 1 ELISA Kit; Alpha amylase ELISA Kit; Alpha-amylase 1 ELISA Kit; AMY1 ELISA Kit; AMY1_HUMAN ELISA Kit; AMY1C ELISA Kit; AMY2 ELISA Kit; AMY2B ELISA Kit; AMY3 ELISA Kit; amylase; alpha 1A (salivary) ELISA Kit; Amylase; alpha 1B (salivary) ELISA Kit; amylase; alpha 1C (salivary) ELISA Kit; amylase; salivary; alpha-1A ELISA Kit; amylase; salivary; alpha-1B ELISA Kit; amylase; salivary; alpha-1C ELISA Kit; amylase; salivary; C ELISA Kit; glycogenase ELISA Kit; HXA ELISA Kit; PA ELISA Kit; Salivary alpha-amylase ELISA Kit; salivary amylase alpha 1A ELISA Kit; salivary amylase alpha 1B ELISA Kit; salivary amylase alpha 1C ELISA Kit

AMY1

Homo sapiens (Human)

serum, saliva, tissue homogenates

1.95 IU/mL

1-5h

50-100ul

450 nm

Metabolism

quantitative

Sandwich

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of human AMY1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		81-93
1:2		Average %		92
		Range %		89-96
1:4		Average %		90
		Range %		84-96
1:8		Average %		93
		Range %		86-98

The recovery of human AMY1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		96-107

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml OD1 OD2 Average Corrected 500 2.991 2.789 2.890 2.788 250 2.607 2.604 2.606 2.504 125 2.254 2.213 2.234 2.132 62.5 1.682 1.569 1.626 1.524 31.2 0.931 0.924 0.928 0.826 15.6 0.522 0.513 0.518 0.416 7.8 0.291 0.288 0.290 0.188 0 0.106 0.098 0.102

3-5 working days