Human alpha-L-fucosidase,AFU ELISA Kit

Human alpha-L-fucosidase,AFU ELISA Kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

人αL岩藻糖苷酶(AFU)酶联免疫试剂盒
货号：

CSB-E08348h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品描述：

人αL岩藻糖苷酶(AFU)酶联免疫试剂盒（CSB-E08348h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FUCA2含量。FUCA2 是一个靶点。其相关它在机体特定生理过程中可能发挥重要作用。研究机制上，科学家可能会从基因表达调控、蛋白功能等角度展开研究，探究其在疾病发生发展中的作用，以寻找潜在治疗靶点和干预措施。试剂盒检测范围为31.25 μU/mL-2000 μU/mL，适用于体外科研实验中AFU的精准定量分析；可为肝脏病理机制研究、肿瘤标志物筛选、代谢性疾病模型构建等基础科研提供可靠数据支持；适用于实验室批量检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Alpha L fucosidase 2 ELISA Kit; Alpha L fucosidase fucohydrolase 2 ELISA Kit; Alpha-L-fucosidase 2 ELISA Kit; Alpha-L-fucoside fucohydrolase 2 ELISA Kit; dJ20N2.5 ELISA Kit; FUCA 2 ELISA Kit; FUCA2 ELISA Kit; FUCO2 ELISA Kit; FUCO2_HUMAN ELISA Kit; Fucosidase alpha L 2 plasma ELISA Kit; Fucosidase; alpha-L; plasma ELISA Kit; MGC1314 ELISA Kit; Plasma alpha L fucosidase ELISA Kit; Plasma alpha-L-fucosidase ELISA Kit; RP1 20N2.5 ELISA Kit
缩写：

FUCA2
Uniprot No.：

Q9BTY2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 μU/mL-2000 μU/mL
灵敏度：

7.8 μU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AFU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	88
	Range %	81-95
1:40	Average %	91
	Range %	86-97
1:80	Average %	97
	Range %	91-102
1:160	Average %	102
	Range %	97-107

回收率：

The recovery of human AFU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-110
EDTA plasma (n=4)	93	85-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µU/ml	OD1	OD2	Average	Corrected
2000	2.720	2.546	2.633	2.546
1000	2.235	2.200	2.218	2.131
500	1.554	1.633	1.594	1.507
250	0.821	0.845	0.833	0.746
125	0.471	0.445	0.458	0.371
62.5	0.311	0.302	0.307	0.220
31.25	0.185	0.188	0.187	0.100
0	0.084	0.090	0.087

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Evaluating new biomarkers for diabetic nephropathy: Role of α2-macroglobulin, podocalyxin, α-L-fucosidase, retinol-binding protein-4, and cystatin C JJ Li,World journal of diabetes,2024

功能：

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
基因功能参考文献：
1. Genetic polymorphisms within the FUCA2 and IL18 gene regions are also associated with diastolic function in SCD, likely by affecting expression levels of the genes PMID: 27636371
2. serum alpha-L-fucosidase was identified as a useful marker for close monitoring of patients during post-treatment follow-up PMID: 18521898
3. Findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for diagnosis and therapy of H. pylori-related diseases. PMID: 19666478
亚细胞定位：

Secreted.
蛋白家族：

Glycosyl hydrolase 29 family
数据库链接：

HGNC: 4008

OMIM: 136820

KEGG: hsa:2519

STRING: 9606.ENSP00000002165

UniGene: Hs.17680

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.