Human alcohol dehydrogenase,ADH ELISA Kit

Human alcohol dehydrogenase,ADH ELISA Kit

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中文名称：

人乙醇脱氢酶(ADH)酶联免疫试剂盒
货号：

CSB-E11855h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人乙醇脱氢酶(ADH)酶联免疫试剂盒（CSB-E11855h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的ADH1A含量。ADH1A是一个重要靶点。它在乙醇代谢等生理过程中发挥关键作用。研究机制方面，聚焦于其基因表达调控及蛋白功能。通过探究它与疾病的关联，如酒精性肝病等，为开发针对性治疗药物和干预策略提供理论依据和研究方向。试剂盒检测范围为0.312 mU/mL-20 mU/mL，适用于酒精代谢机制研究、肝损伤模型评估、药物或化合物对ADH活性调控作用分析等科研场景，特别针对体外培养细胞或动物实验样本中的ADH活性检测需求开发，为代谢组学、毒理学及肝脏病理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

ADH alpha subunit ELISA Kit; ADH ELISA Kit; ADH1 ELISA Kit; ADH1A ELISA Kit; ADH1A_HUMAN ELISA Kit; Alcohol dehydrogenase 1 (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; Alcohol dehydrogenase 1A (class I), alpha polypeptide ELISA Kit; Alcohol dehydrogenase 1A ELISA Kit; Alcohol dehydrogenase subunit alpha ELISA Kit; Aldehyde reductase ELISA Kit
缩写：

ADH1A
Uniprot No.：

P07327
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

0.312 mU/mL-20 mU/mL
灵敏度：

0.078 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-87
1:2	Average %	102
	Range %	97-107
1:4	Average %	94
	Range %	90-99
1:8	Average %	86
	Range %	83-89

回收率：

The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	99-105
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
20	2.294	2.156	2.225	2.107
10	1.778	1.698	1.738	1.620
5	1.253	1.240	1.247	1.129
2.5	0.797	0.775	0.786	0.668
1.25	0.485	0.468	0.477	0.359
0.625	0.362	0.375	0.369	0.251
0.312	0.256	0.262	0.259	0.141
0	0.119	0.117	0.118

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

基因功能参考文献：
1. High ADH expression is associated with Prostate Cancer. PMID: 28870918
2. Results suggest a potential role of alcohol dehydrogenase (ADH) class I as a marker for renal cell carcinoma (RCC). PMID: 27086037
3. Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer. PMID: 24762066
4. Unde-rexpression of the ADH1 gene, which influences the transformation of the extracellular matrix, plays a probable role in the etiology of uterine fibroid. PMID: 23891545
5. high levels of ADH1 transcription are implicated in fluconazole resistance in C. albicans and that the mRNA expression levels of ADH1 are positively correlated with those of CDR1, CDR2 and FLU1. PMID: 23769565
6. common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
7. High ADH1 is associated with endometrial cancer. PMID: 20872569
8. ADH1A variation predisposes to personality traits and substance dependence PMID: 19526455
9. Structural aspects that influence dimer-tetramer formation. PMID: 12081471
10. GATA-2 and HNF-3beta regulate the human alcohol dehydrogenase 1A (ADH1A) gene. PMID: 16153155
11. The oxidative pathway of ethanol metabolism via ADH and ALDH does not play a role in pancreatic carcinogenesis. PMID: 17632320
12. Alleles of ADH7 SNPs were associated with the early stages of alcohol metabolism, with additional effects in the ADH1A, ADH1B and ADH4 regions. PMID: 19193628
13. Observational study of healthcare-related. (HuGE Navigator) PMID: 15279067
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亚细胞定位：

Cytoplasm.
蛋白家族：

Zinc-containing alcohol dehydrogenase family
数据库链接：

HGNC: 249

OMIM: 103700

KEGG: hsa:124

STRING: 9606.ENSP00000209668

UniGene: Hs.654433

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.