Human activated coagulation factor Ⅻ,FⅫa ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人活化凝血因子Ⅻ(FⅫa)酶联免疫试剂盒（CSB-E09232h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FⅫa含量。FⅫa是凝血因子Ⅻ激活后形成的丝氨酸蛋白酶。其参与内源性凝血途径启动。研究发现它不仅在凝血中起作用，还与炎症、血栓形成等相关。对FⅫa作用机制的研究，有助于开发新的抗血栓药物，且可能减少出血风险。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒通过高特异性抗体对靶标进行捕获与检测，适用于体外研究FⅫa在凝血机制、血管通透性调控或相关疾病动物模型中的动态变化，可为心血管疾病、免疫炎症反应等领域的科研实验提供定量分析支持。该产品仅用于科学研究，不适用于其他用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

FⅫa
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FⅫa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	111
	Range %	105-116
1:2	Average %	95
	Range %	88-102
1:4	Average %	96
	Range %	90-100
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human FⅫa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	108	103-114

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.