Human Tumor necrosis factor-inducible gene 6 protein(TNFAIP6) ELISA kit
产品详情
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产品描述：
人肿瘤坏死因子诱导基因6蛋白(TNFAIP6)酶联免疫试剂盒（CSB-EL023959HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFAIP6含量。TNFAIP6 靶点具有重要生物学意义。它可能参与炎症、组织修复等生理过程。相关研究聚焦于其在疾病发生发展中的作用机制，探索其作为治疗靶点的潜力，如在炎症性疾病等领域，为开发针对性药物提供理论基础。试剂盒检测范围为47 ng/ml- 3000 ng/ml，适用于基础科研中探索TNFAIP6在炎症性疾病、纤维化病变或肿瘤发展中的表达规律，亦可用于评估体外实验模型中药物干预或基因调控对该靶点的影响。为研究TNFAIP6在病理生理过程中的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：
Hyaluronate binding protein ELISA Kit; Hyaluronate-binding protein ELISA Kit; TNF alpha induced protein 6 ELISA Kit; TNF alpha-induced protein 6 ELISA Kit; TNF stimulated gene 6 protein ELISA Kit; TNF-stimulated gene 6 protein ELISA Kit; TNFAIP 6 ELISA Kit; Tnfaip6 ELISA Kit; TSG 6 ELISA Kit; TSG-6 ELISA Kit; TSG6_HUMAN ELISA Kit; Tumor necrosis factor alpha induced protein 6 ELISA Kit; Tumor necrosis factor alpha inducible protein 6 ELISA Kit; Tumor necrosis factor alpha-induced protein 6 ELISA Kit; Tumor necrosis factor inducible gene 6 protein ELISA Kit; Tumor necrosis factor stimulated gene 6 protein ELISA Kit; Tumor necrosis factor-inducible gene 6 protein ELISA Kit
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缩写：
TNFAIP6
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Uniprot No.：
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种属：
Homo sapiens (Human)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
47 ng/ml - 3000 ng/ml
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灵敏度：
11.75 ng/ml
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cancer
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFAIP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) | |
1:1 | Average % | 99 | |
Range % | 92-106 | |
1:2 | Average % | 85 | |
Range % | 81-92 | |
1:4 | Average % | 89 | |
Range % | 84-94 | |
1:8 | Average % | 95 | |
Range % | 88-101 | |
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回收率：
The recovery of human TNFAIP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 94 | 89-100 | |
EDTA plasma (n=4) | 88 | 81-95 | |
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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ng/ml | OD1 | OD2 | Average | Corrected | |
3000 | 2.715 | 2.634 | 2.674 | 2.546 | |
1500 | 2.032 | 2.167 | 2.099 | 1.972 | |
750 | 1.329 | 1.278 | 1.303 | 1.175 | |
375 | 0.786 | 0.749 | 0.767 | 0.640 | |
187.5 | 0.427 | 0.411 | 0.419 | 0.291 | |
94 | 0.294 | 0.278 | 0.286 | 0.158 | |
47 | 0.227 | 0.217 | 0.222 | 0.094 | |
0 | 0.131 | 0.125 | 0.128 | | |
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本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TNFAIP6 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TNFAIP6 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：
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货期：
3-5 working days
产品评价
样品类型：血浆（抗凝剂） EDTA
样品信息：人
稀释比：没有稀释
产品评价： ELISA结果正常组和患病组有明显差异
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