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  4. Human Troponin I, fast skeletal muscle(TNNI2) ELISA kit

Human Troponin I, fast skeletal muscle(TNNI2) ELISA kit

说明书下载
  • 中文名称：
    人肌钙蛋白I，骨骼肌快肌(TNNI2)酶联免疫试剂盒
  • 货号：
    CSB-EL024012HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人肌钙蛋白I，骨骼肌快肌(TNNI2)酶联免疫试剂盒（CSB-EL024012HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的TNNI2含量。TNNI2 即心肌肌钙蛋白 I2，它作为心肌收缩调节的关键蛋白，与心肌功能紧密相关。在研究机制上，其异常表达或突变会影响心肌收缩舒张功能，在心肌疾病发生发展中起重要作用，是心血管疾病研究的潜在靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于肌肉发育、运动损伤模型、肌病机制等基础研究领域。科研人员可通过定量分析不同生理或病理状态下骨骼肌损伤标志物TNNI2的动态变化，为探索肌肉再生机制或药物干预效果提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AMCD 2B ELISA Kit; AMCD2B ELISA Kit; DA 2B ELISA Kit; DA2B ELISA Kit; fast skeletal muscle ELISA Kit; Fast twitch skeletal muscle troponin I ELISA Kit; fast-twitch isoform ELISA Kit; FSSV ELISA Kit; fsTnI ELISA Kit; TNNI 2 ELISA Kit; Tnni2 ELISA Kit; TNNI2_HUMAN ELISA Kit; tro ELISA Kit; Troponin I ELISA Kit; Troponin I fast skeletal muscle ELISA Kit; Troponin I fast twitch 2 ELISA Kit; Troponin I fast twitch isoform ELISA Kit; Troponin I fast twitch skeletal muscle isoform ELISA Kit; Troponin I skeletal fast ELISA Kit; Troponin I type 2 (skeletal fast) ELISA Kit; Troponin I type 2 ELISA Kit
  • 缩写：
    TNNI2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma,tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TNNI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    85

    Range %

    81-90

    1:2

    Average %

    93

    Range %

    88-99

    1:4

    Average %

    92

    Range %

    88-97

    1:8

    Average %

    91

    Range %

    86-96

  • 回收率：

    The recovery of human TNNI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    87-97

    EDTA plasma (n=4)

    98

    91-104

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    400

    2.236

    2.334

    2.285

    2.127

    200

    1.642

    1.667

    1.655

    1.497

    100

    1.227

    1.288

    1.257

    1.100

    50

    0.857

    0.829

    0.843

    0.686

    25

    0.610

    0.621

    0.616

    0.458

    12.5

    0.429

    0.453

    0.441

    0.283

    6.25

    0.244

    0.234

    0.239

    0.081

    0

    0.160

    0.155

    0.158

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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TNNI2 antibodies

TNNI2 Proteins

相关问答

■ 常见问题解答
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
d. The customer is asking about cross-reactivity with homologous proteins, espessialy with cTnI. Did you test it? Can you please provide such kind of information with data?

A:
Thanks for your inquiry!
CSB-EL024012hu
This kit is designed against full length protein.
Capture antibody is goat polyclonal antibody.
Detection antibody is goat polyclonal antibody.
Immunogen of capture antibody is 293 cell derived recombinant protein, full length.
Immunogen of detection antibody is 293 cell derived recombinant protein, partial.
Standard is 293 cell derived recombinant protein.There is no cross reactivity with cTnI.
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靶点详情

  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能参考文献：
    1. Mutations in genes encoding cardiac troponin I (TNNI3) and cardiac troponin T (TNNT2) caused altered troponin protein stoichiometry in patients with dilated cardiomyopathy. TNNI3p.98trunc resulted in haploinsufficiency, increased Ca(2+) -sensitivity and reduced length-dependent activation. TNNT2p.K217del caused increased passive tension. PMID: 28436080
    2. TNNI2 gene missense mutation is associated with distal arthrogryposis type 1. PMID: 26374086
    3. report the first TNNI2 mutation in classical FSS and describe an atypical adult FSS case with only facial contractures resulting from somatic mosaicism PMID: 23850728
    4. Molecular genetic investigations revealed pathogenic mutations in MYH3, TPM2, and TNNI2 in one sporadic and 19 familial cases of distal arthrogryposis. PMID: 22519952
    5. Atrial re-expression of ssTnI during paroxysmal atrial fibrillation in patients does not seem to be part of the process of AF-associated cardiomyocyte dedifferentiation but seems to result from transient cardiomyocyte stress at the onset of AF. PMID: 15289369
    6. study found a novel TNNI2 mutation in a Chinese family with distal arthrogryposis type 2B PMID: 16802141
    7. Implantation of stable human clone expressing fast-twitch skeletal muscle troponin I in female BALB/c nude mice inhibits primary tumor growth and suggests that grafts are self-inhibitory by halting angiogenesis. PMID: 17393089
    8. These findings reveal a new function for TNNI2 as a co-activator of ERRalpha. PMID: 18331830
    9. Data found that fTnI normally associated with fast twitch skeletal muscle were present at significant levels in the thoracic aorta, and that fTnI transcripts were expressed in the smooth muscle layer of mouse blood vessels of all sizes. PMID: 18548613
    10. Gene expression revealed up-regulation of pro-angiogenic (PGF), anti-apoptotics (BAG-1, BCL-2), heart development (TNNT2, TNNC1) and extracellular matrix remodelling (MMP-2, MMP-7) genes in SM. PMID: 18805052

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  • 相关疾病：
    Arthrogryposis, distal, 2B (DA2B)
  • 蛋白家族：
    Troponin I family
  • 数据库链接：

    HGNC: 11946

    OMIM: 191043

    KEGG: hsa:7136

    STRING: 9606.ENSP00000252898

    UniGene: Hs.523403

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |