Human Transmembrane protein 95(TMEM95) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人跨膜蛋白95(TMEM95)酶联免疫试剂盒（CSB-EL023899HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TMEM95含量。人跨膜蛋白95（TMEM95）是一种精子膜蛋白，其敲除在小鼠中会导致男性特异性不育表型。研究发现，TMEM95与卵子膜结合，促进精子与卵子的膜融合，其作用机制尚待进一步研究。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒通过高特异性配对抗体实现精准检测，可兼容多种生物样本类型，适用于基础科研中探索TMEM95在生殖系统生理功能、疾病模型中的表达变化，或结合其他分子标记物解析相关调控机制。其宽泛的检测范围可满足不同浓度样本的定量需求，为体外实验、动物模型或组织样本的蛋白表达研究提供可靠工具，助力生殖健康、发育生物学等领域的分子机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

TMEM95 ELISA Kit; UNQ9390/PRO34281 ELISA Kit; Transmembrane protein 95 ELISA Kit
缩写：

TMEM95
Uniprot No.：

Q3KNT9
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM95 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	100
	Range %	95-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	89-96

回收率：

The recovery of human TMEM95 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Sperm protein required for fusion of sperm with the egg membrane during fertilization.
亚细胞定位：

Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein.
数据库链接：

HGNC: 27898

OMIM: 617814

KEGG: hsa:339168

STRING: 9606.ENSP00000331466

UniGene: Hs.259432