Human Thromboxane B2,TX-B2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人血栓素B2(TXB2)酶联免疫试剂盒（CSB-E08046h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TX-B2含量。TX-B2，即血栓素B2，是一种由血小板合成的脂质过氧化物，参与调节血管收缩和血小板聚集。其背景研究涉及血栓形成和心血管疾病。研究机制主要关注TX-B2在血栓形成过程中的作用，以及如何通过调节TX-B2水平来预防和治疗相关疾病。试剂盒检测范围为9.38 pg/mL-600 pg/mL，该产品广泛应用于心血管疾病机制研究、抗血小板药物药效评估、炎症信号通路探索等科研领域，例如通过检测阿司匹林干预后血小板释放的 TXB2 水平变化，或分析动脉粥样硬化模型动物组织中的血栓素代谢动态本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

TX-B2
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

9.38 pg/mL-600 pg/mL
灵敏度：

2.34 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TX-B2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	80-105
1:2	Average %	95
Range %	85-108
1:4	Average %	106
Range %	90-112
1:8	Average %	95
Range %	80-110

回收率：

The recovery of human TX-B2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	80-110
EDTA plasma (n=4)	98	90-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.