Human Thiosulfate sulfurtransferase(TST) ELISA kit

Human Thiosulfate sulfurtransferase(TST) ELISA kit

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中文名称：

人硫代硫酸硫转移酶(TST)酶联免疫试剂盒
货号：

CSB-EL025197HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人硫代硫酸硫转移酶(TST)酶联免疫试剂盒（CSB-EL025197HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TST含量。人硫代硫酸硫转移酶（TST）是一种线粒体酶，主要功能是催化硫代硫酸盐和氰根离子转化为亚硫酸盐和硫氰酸盐，参与代谢和解毒过程。它在氧化还原反应中起关键作用，可能影响蛋白质稳定性和折叠，并在翻译过程中对tRNA核苷的转硫化起重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研场景下探究TST在硫代谢通路中的功能机制、氧化应激相关疾病模型中的表达动态，或用于药物研发中化合物对TST活性影响的评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

GlpE ELISA Kit; RDS ELISA Kit; Rhodanese ELISA Kit; Thiosulfate sulfurtransferase ELISA Kit; Thiosulfate sulfurtransferase GlpE ELISA Kit; THTR_HUMAN ELISA Kit; TST ELISA Kit
缩写：

TST
Uniprot No.：

Q16762
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	100
	Range %	92-110
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of human TST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.504	2.511	2.508	2.352
500	2.131	2.052	2.092	1.936
250	1.415	1.333	1.374	1.218
125	0.759	0.744	0.752	0.596
62.5	0.541	0.523	0.532	0.376
31.2	0.337	0.323	0.330	0.174
15.6	0.251	0.244	0.248	0.092
0	0.159	0.153	0.156

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
基因功能参考文献：
1. TST mRNA expression in adipose tissue correlated positively with insulin sensitivity in adipose tissue and negatively with fat mass. PMID: 27270587
2. show that polymorphic variations that are distant from the active site differentially modulate the sulfurtransferase activity of human rhodanese to cyanide versus sulfite PMID: 26269602
3. Loss of Thiosulfate Sulfurtransferase is associated with decreased mucosal sulfide detoxification capacity in patients with Crohn's disease PMID: 23399736
4. silencing of the rhodanese gene caused not only a proportional decrease of 5 S rRNA import but also a general inhibition of mitochondrial translation, indicating the functional importance of the imported 5 S rRNA inside the organelle. PMID: 20663881
5. Proteomics was used to study colonic epithelial aging, for differential proteins in the human normal colonic epithelial tissues from young and old people. Rack1, EF-Tu and Rhodanese, three validated differential proteins, were further investigated. PMID: 20099848
6. A partially gluconoylated N-terminus of the His6-tagged molybdenum cofactor synthesis 3 (MOCS3)-rhodanese-like domain is identified which results in a heterogeneity of the protein but does not influence sulfurtransferase activity. PMID: 15910006
7. The USP8 recognition domain of NRDP1 has a novel protein fold that interacts with a conserved peptide loop of the rhodanese domain. PMID: 17035239
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亚细胞定位：

Mitochondrion matrix.
数据库链接：

HGNC: 12388

OMIM: 180370

KEGG: hsa:7263

STRING: 9606.ENSP00000249042

UniGene: Hs.474783

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.