Human Tenascin-N(TNN) ELISA kit

Human Tenascin-N(TNN) ELISA kit

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中文名称：

人肌腱蛋白N(TNN)酶联免疫试剂盒
货号：

CSB-EL024008HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人肌腱蛋白N(TNN)酶联免疫试剂盒（CSB-EL024008HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TNN含量。人肌腱蛋白N（TNN）是一种重要的多肽糖蛋白，在生物体内发挥着多种生物学功能，如组织或细胞黏附、调控细胞迁移和生存等。其研究机制涉及基因表达、结构和功能等多方面，对理解其生物学功能和疾病发生具有重要意义。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外培养细胞功能研究、动物模型组织分析以及生物体液样本中TNN的动态监测，为探索其在炎症反应、纤维化疾病或肿瘤发生发展中的分子机制提供可靠工具，支持科研工作者开展基础医学研究中的蛋白表达定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

TNN ELISA Kit; TNW ELISA Kit; Tenascin-N ELISA Kit; TN-N ELISA Kit; Tenascin-W ELISA Kit; TN-W ELISA Kit
缩写：

TNN
Uniprot No.：

Q9UQP3
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-99
1:2	Average %	102
	Range %	97-105
1:4	Average %	94
	Range %	88-98
1:8	Average %	98
	Range %	93-102

回收率：

The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	92	86-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.022	2.001	2.012	1.855
5	1.733	1.812	1.773	1.616
2.5	1.307	1.288	1.298	1.141
1.25	0.989	0.973	0.981	0.824
0.625	0.673	0.666	0.670	0.513
0.312	0.466	0.454	0.460	0.303
0.156	0.301	0.312	0.307	0.150
0	0.156	0.158	0.157

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1. Involved in neurite outgrowth and cell migration in hippocampal explants. During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells. Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells.
基因功能参考文献：
1. This study demonstrated that the genetic defect was totally or partly clarified in 21 patients with nine of them having potential disease-causing mutations in TTN. PMID: 26627873
2. Results show that tenascin-W acts as a niche component for breast cancer metastasis to bone by supporting cell migration and cell proliferation of the cancer cells. PMID: 25868708
3. The expression of tenascin-W is dependent on p38MAPK and JNK signaling pathways in mammary tumors. (tenascin-W) PMID: 15592496
4. data imply that tenascin-W expression in the activated tumor stroma facilitates tumorigenesis by supporting the migratory behavior of breast cancer cells PMID: 17909022
5. results reveal a clear association between elevated levels of tenascin-W and the presence of colorectal cancer and breast cancer PMID: 18306355
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Tenascin family
组织特异性：

Not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested. In brain tumors, detected around the endothelial cell layer of the clood vessel
数据库链接：

HGNC: 22942

OMIM: 617472

KEGG: hsa:63923

STRING: 9606.ENSP00000239462

UniGene: Hs.156369

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.