Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人酒石酸盐抵抗的酸性磷酸酶（TRAP）酶联免疫检测试剂盒（货号：CSB-E13415h），基于双抗体夹心法定量原理，适用于血清、血浆及组织匀浆样本中TRAP蛋白含量的精准检测。TRAP作为酸性磷酸酶家族成员，在破骨细胞介导的骨吸收过程中发挥关键作用，其表达水平与骨代谢异常、骨质疏松等病理机制密切相关，是骨生物学研究的重要标志物。试剂盒检测范围覆盖0.47 ng/mL至30 ng/mL，通过预包被高亲和力捕获抗体与标记检测抗体的协同作用，实现目标蛋白的特异性识别与信号放大，实验流程包含标准品梯度稀释、样本孵育、显色反应等标准化步骤。该试剂盒适用于体外骨代谢研究、破骨细胞活性评估、骨相关疾病机制探索以及药物干预效果评价等科研领域，其高灵敏度的检测性能可有效支持微量样本中TRAP的动态变化分析，为骨生物学、细胞信号通路等基础研究提供可靠工具。
别名：

N/A
缩写：

TRAP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.47 ng/mL-30 ng/mL
灵敏度：

0.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-96
1:2	Average %	97
	Range %	95-100
1:4	Average %	94
	Range %	90-98
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of human TRAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	89	84-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.