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  4. Human T-cell surface protein tactile(CD96) ELISA kit

Human T-cell surface protein tactile(CD96) ELISA kit

说明书下载
  • 中文名称：
    人T细胞表面触觉蛋白(CD96)酶联免疫试剂盒
  • 货号：
    CSB-EL004971HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人T细胞表面触觉蛋白（CD96）酶联免疫检测试剂盒（货号CSB-EL004971HU），采用双抗体夹心法定量检测血清、血浆、组织匀浆及细胞裂解液等样本中的CD96蛋白水平。CD96作为免疫检查点分子，主要表达于活化T细胞和NK细胞表面，通过介导细胞间黏附作用参与免疫调控，其表达水平与肿瘤免疫逃逸、自身免疫性疾病等研究密切相关。本试剂盒检测灵敏度范围为0.156-10 ng/mL，可在低浓度样本中实现精准定量，适用于体外实验中对CD96蛋白表达的动态监测，例如肿瘤微环境研究、免疫细胞功能评估或抗体药物作用机制探索等领域。实验操作基于预包被特异性抗体的酶标板，通过生物素标记检测抗体与链霉亲和素-HRP系统的级联反应实现信号放大，确保检测的高特异性和稳定性。该试剂盒为研究CD96在免疫调控网络中的功能及其与疾病关联性提供了可靠工具。
  • 别名：
    CD96; T-cell surface protein tactile; Cell surface antigen CD96; T cell-activated increased late expression protein; CD antigen CD96
  • 缩写：
    CD96
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CD96 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 96
    Range % 92-100
    1:4 Average % 104
    Range % 100-112
    1:8 Average % 94
    Range % 90-100
  • 回收率：
    The recovery of human CD96 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-93
    EDTA plasma (n=4) 93 88-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.045 1.972 2.009 1.836
    5 1.502 1.581 1.542 1.369
    2.5 1.104 1.054 1.079 0.906
    1.25 0.711 0.727 0.719 0.546
    0.625 0.510 0.505 0.508 0.335
    0.312 0.361 0.349 0.355 0.182
    0.156 0.294 0.286 0.290 0.117
    0 0.175 0.170 0.173 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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CD96 antibodies

CD96 Proteins

靶点详情

  • 功能：
    May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
  • 基因功能参考文献：
    1. Lower CD96 expression was also observed in human IL-9(+) compared with IFN-gamma(+) T cells PMID: 29531070
    2. Blocking CD96 or TIGIT with mAbs has been shown to improve tumor control in mice. PMID: 27620276
    3. CD96 and CD123 are expressed in bone marrow cells of patients with myelodysplastic syndromes PMID: 26642704
    4. In the present study we analyzed the expression of four cell surface antigens relevant to human hematopoiesis-CD90, CD96, CD117, and CD123-in bone marrow from pediatric acute myeloid leukemia patients and normal control subjects. PMID: 24751333
    5. down-regulation of CD96 is an important aspect of HIV-1 pathogenesis and differential expression is related to cell effector functions and HIV-1 disease course. PMID: 23272144
    6. CD96 could serve as a target structure for effector cell-mediated lysis. PMID: 22879978
    7. The positive expression of CD96 on bone marrow hematopoietic stem cells in patients with acute leukemia may be associated with primary drug resistance, relapse and progression. PMID: 21729528
    8. CD96 promotes natural killer (NK) cell adhesion to target cells expressing the poliovirus receptor (PVR), stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. PMID: 15034010
    9. CD96 is a cell surface marker present on many acute myeloid leukemia leukemic stem cells PMID: 17576927
    10. CD96-driven adhesion to CD155 may be crucial in developmental processes PMID: 19056733

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  • 相关疾病：
    C syndrome (CSYN)
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
  • 数据库链接：

    HGNC: 16892

    OMIM: 211750

    KEGG: hsa:10225

    STRING: 9606.ENSP00000283285

    UniGene: Hs.142023

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |