Human Statherin(STATH) ELISA kit

Human Statherin(STATH) ELISA kit

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中文名称：

人富酪蛋白(STATH)酶联免疫试剂盒
货号：

CSB-EL022817HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人富酪蛋白（STATH）酶联免疫检测试剂盒，采用双抗体夹心法原理进行定量分析，可精准检测血清、血浆、组织匀浆及唾液样本中的STATH蛋白含量，检测灵敏度范围为78-5000 ng/mL。STATH是一种主要由唾液腺分泌的钙结合蛋白，在维持口腔矿物质平衡、抑制羟基磷灰石结晶过度沉积中发挥重要作用，其表达水平与牙釉质发育及口腔健康密切相关。该试剂盒基于高特异性抗体对靶标进行捕获与检测，操作流程标准化，适用于科研领域中对唾液成分分析、口腔疾病机制研究、牙釉质生物矿化过程探索等方向，尤其可为龋齿风险因素评估、口腔黏膜保护机制等基础研究提供可靠数据支持。试剂盒支持多种生物样本类型检测，配备优化的反应缓冲体系，确保实验结果的稳定性和重复性，满足体外实验中对STATH蛋白表达水平的定量分析需求。
别名：

STAT_HUMAN ELISA Kit; STATH ELISA Kit; Statherin ELISA Kit; Statherin precursor ELISA Kit; STR ELISA Kit
缩写：

STATH
Uniprot No.：

P02808
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, saliva
检测范围：

78 ng/mL-5000 ng/mL
灵敏度：

19.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human STATH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	95
	Range %	91-104
1:4	Average %	86
	Range %	82-92
1:8	Average %	96
	Range %	92-101

回收率：

The recovery of human STATH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	99	94-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5000	2.751	2.651	2.701	2.547
2500	1.829	1.729	1.779	1.625
1250	1.087	1.067	1.077	0.923
625	0.589	0.579	0.584	0.430
312.5	0.382	0.372	0.377	0.223
156	0.265	0.255	0.260	0.106
78	0.209	0.207	0.208	0.054
0	0.154	0.153	0.154

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Correlation of Salivary Statherin and Calcium Levels with Dental Calculus Formation: A Preliminary Study Deepak Gowda Sadashivappa Patee.et al,International Journal of Dentistry,2017

功能：

Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface.
基因功能参考文献：
1. The total protein and statherin in the in-vivo AEP were different between eroded and non-eroded tooth surfaces of the same patient PMID: 28837608
2. effects of statherin on intracellular calcium level and its subsequent related molecular alterations would give us new pathogenic aspect in oral carcinogenesis PMID: 25128293
3. phenylalanine orientations in statherin bound to hydroxyapatite surfaces PMID: 22563672
4. Data show that the full characterization of the statherin peptides generated facilitates the elucidation of their novel functional roles in the oral and gastro-intestinal environment. PMID: 20731414
5. insight into the molecular interactions of statherin with hydroxyapatite surfaces PMID: 19678690
6. inhibits calcium phosphate precipitation PMID: 12060866
7. results suggest that a layer rich in statherin forms at the interface of saliva and air, and that the surface rheology developed is dependent upon protein interactions mediated by calcium PMID: 15769251
8. In conclusion, statherin induces transition to yeast of Candida albicans hyphae and may thus contribute to the oral defense against candidiasis. PMID: 19799638
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亚细胞定位：

Secreted.
蛋白家族：

Histatin/statherin family
组织特异性：

Secreted by parotid and submandibular glands.
数据库链接：

HGNC: 11369

OMIM: 184470

KEGG: hsa:6779

STRING: 9606.ENSP00000246895

UniGene: Hs.654495

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.