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  4. Human Spondin-2 ELISA Kit

Human Spondin-2 ELISA Kit

文献引用 (2) 说明书下载
  • 中文名称：
    人Spondin-2酶联免疫试剂盒
  • 货号：
    CSB-E14967h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人Spondin-2（R-spondin2）是一种分泌型蛋白，属于R-spondin家族成员，通过调控Wnt/β-catenin信号通路参与胚胎发育、组织再生及干细胞微环境调控等生物学过程。本试剂盒（货号：CSB-E14967h）基于双抗体夹心法的定量检测原理，可精准分析血清、血浆、脑脊液及细胞培养上清等样本中的Spondin-2蛋白水平，检测灵敏度为3.12 ng/mL，线性检测范围为3.12-200 ng/mL。试剂盒包含预包被特异性抗体的酶标板、标准品及检测所需试剂，操作流程约4小时内完成。该产品适用于基础科研领域，如探究Spondin-2在肿瘤发生、神经退行性疾病或组织修复中的分子机制，也可用于体外细胞模型中蛋白分泌水平的动态监测，为疾病相关生物标志物研究及信号通路调控分析提供高效工具。
  • 别名：
    Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit; DIL 1 ELISA Kit; DIL-1 ELISA Kit; DIL1 ELISA Kit; DKFZp686G21139 ELISA Kit; FLJ16313 ELISA Kit; FLJ22401 ELISA Kit; FLJ34460 ELISA Kit; M spondin ELISA Kit; Mindin ELISA Kit; ORF Names: ELISA Kit; SPON 2 ELISA Kit; Spon2 ELISA Kit; SPON2_HUMAN ELISA Kit; Spondin 2 ELISA Kit; Spondin 2 extracellular matrix protein ELISA Kit; Spondin-2 ELISA Kit
  • 缩写：
    SPON2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cerebrospinal fluid (CSF), cell culture supernates
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 97  
    Range % 91-100  
    1:4 Average % 97  
    Range % 92-104  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human spondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    200 1.825 1.897 1.861 1.744  
    100 1.414 1.435 1.425 1.308  
    50 0.996 0.981 0.989 0.872  
    25 0.692 0.683 0.688 0.571  
    12.5 0.423 0.458 0.441 0.324  
    6.25 0.295 0.280 0.288 0.171  
    3.12 0.206 0.216 0.211 0.094  
    0 0.116 0.118 0.117 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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SPON2 antibodies

SPON2 Proteins

靶点详情

  • 功能：
    Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
  • 基因功能参考文献：
    1. Egr-1 regulates mindin expression by directly binding to the mindin promoter; mindin suppresses colon cancer progression by blocking angiogenesis. PMID: 28991232
    2. Spondin-2 overexpression contributes to tumor aggressiveness and prognosis in gastric cancer. PMID: 28060752
    3. SPON2 is a transcriptional target of the metastasis gene MACC1. SPON2 induces cell motility in vitro and CRC metastasis in mice. In patients, SPON2 serves as prognostic indicator for CRC metastasis and survival, and might represent a promising target for therapeutic approaches. PMID: 26686083
    4. Data suggest that spondin-2 could be an independent diagnostic and prognostic biomarker of colon cancer. PMID: 25945835
    5. Mindin protects against vascular hyperplasia by suppression of abnormal VSMC proliferation, migration and phenotypic switching in an AKT-dependent manner. PMID: 25751394
    6. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in prostate cancer (PCa). SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. PMID: 22615945
    7. mindin serves as a novel mediator that protects against cardiac hypertrophy and the transition to heart failure by blocking AKT/GSK3beta and TGF-beta1-Smad signalling. PMID: 21632881
    8. High Mindin is associated with diabetic nephropathy in type 2 diabetes. PMID: 21098016
    9. Spondin 2, which is regulated by T(3), has an important role in cell invasion, cell migration, and hepatoma tumor progression. PMID: 19903741
    10. The structure of the F-spondin domain of mindin was determined at 1.8-A resolution. PMID: 19153605

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Expressed in normal lung tissue but not in lung carcinoma cell lines.
  • 数据库链接：

    HGNC: 11253

    OMIM: 605918

    KEGG: hsa:10417

    STRING: 9606.ENSP00000290902

    UniGene: Hs.302963

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |