Human Spondin-1(SPON1) ELISA kit

Human Spondin-1(SPON1) ELISA kit

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中文名称：

人脊椎蛋白-1(SPON1)酶联免疫试剂盒
货号：

CSB-EL022599HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人脊椎蛋白-1（SPON1）酶联免疫试剂盒（货号：CSB-EL022599HU），采用双抗体夹心法原理实现定量检测，适用于科研领域中对血清、血浆及组织匀浆样本中SPON1蛋白水平的分析。SPON1属于脊椎蛋白家族成员，参与细胞黏附和信号传导调控，其表达与神经发育、骨骼系统疾病及肿瘤微环境调控等生物学过程密切相关，尤其在肿瘤血管生成和转移机制研究中备受关注。该试剂盒检测灵敏度为0.156 ng/mL，线性检测范围为0.156-10 ng/mL，可精准检测样本中微量SPON1蛋白的表达动态。科研人员可通过该试剂盒探究SPON1在神经退行性疾病模型中的表达特征，分析其在肿瘤微环境中的调控作用，或评估骨骼发育相关疾病中该蛋白的生物标志物价值。试剂盒操作流程标准化，配套试剂齐全，适用于体外实验中的基础机制研究、疾病模型分析及生物标志物筛选等科研场景，为探索SPON1的分子功能及其在病理生理过程中的作用提供可靠工具。
别名：

SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
缩写：

SPON1
Uniprot No.：

Q9HCB6
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-91
1:2	Average %	86
	Range %	81-92
1:4	Average %	92
	Range %	87-100
1:8	Average %	95
	Range %	88-101

回收率：

The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	93	86-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.504	2.463	2.484	2.365
5	2.006	2.042	2.024	1.905
2.5	1.506	1.479	1.493	1.374
1.25	0.973	0.897	0.935	0.816
0.625	0.511	0.518	0.515	0.396
0.312	0.285	0.268	0.276	0.157
0.156	0.176	0.187	0.182	0.063
0	0.121	0.117	0.119

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

P135 Association of Serum Anti-Muscarinic 3 Antibodies with Gastrointestinal Disease in Systemic Sclerosis E Roblin,/,2024
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功能：

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
基因功能参考文献：
1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
数据库链接：

HGNC: 11252

OMIM: 604989

KEGG: hsa:10418

UniGene: Hs.643864

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.