Human Soluble Elastin Fragments (sELAF) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人可溶性弹性蛋白片段（sELAF）酶联免疫检测试剂盒（货号：CSB-E14110h），专为定量分析血清、血浆及组织匀浆样本中的sELAF含量而设计。sELAF是弹性蛋白降解产生的生物标志物，广泛参与血管弹性调控、组织修复及纤维化进程，其水平变化与心血管疾病、肺部纤维化及皮肤老化等病理生理机制密切相关。CUSABIO试剂盒采用双抗体夹心法原理，定量检测范围覆盖3.12 ng/mL至200 ng/mL，具备高灵敏度和特异性，可稳定检测微量样本中的目标蛋白。适用于基础科研中探究弹性蛋白代谢异常相关疾病模型、药物干预效果评估或生物标记物筛选，例如通过分析心血管疾病患者血清或肺纤维化动物模型组织匀浆中的sELAF水平，揭示其与疾病进展的关联性。试剂盒包含预包被抗体、标准品及优化反应体系，操作便捷，支持科研人员高效开展弹性蛋白代谢调控机制研究。
缩写：

sELAF
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sELAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-100
1:2	Average %	101
	Range %	93-105
1:4	Average %	95
	Range %	88-98
1:8	Average %	91
	Range %	87-94

回收率：

The recovery of human sELAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-102
EDTA plasma (n=4)	87	82-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.