Human Small proline-rich protein 2B(SPRR2B) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人小富脯氨酸蛋白2B(SPRR2B)酶联免疫试剂盒（CSB-EL022613HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SPRR2B含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，支持科研人员在基础研究中探索SPRR2B在皮肤生理病理过程中的作用机制，例如表皮分化异常、皮肤炎症模型或相关疾病分子调控研究，为体外实验提供可靠的蛋白表达定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

SPRR2B ELISA Kit; Small proline-rich protein 2B ELISA Kit; SPR-2B ELISA Kit
缩写：

SPRR2B
Uniprot No.：

P35325
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPRR2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	103
	Range %	99-105
1:4	Average %	93
	Range %	87-97
1:8	Average %	91
	Range %	88-96

回收率：

The recovery of human SPRR2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	90	86-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
基因功能参考文献：
1. Knockdown of the Sprr2b gene or inhibition of SPRR2B phosphorylation attenuates USP7/MDM2 binding and p53 degradation, leading to fibroblasts cell cycle arrest. PMID: 29581288
2. An SNP in SPRR2B was predictive of asthma among white children with eczema from 2 independent populations. PMID: 22374195
3. We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
4. SPR2B transcripts and protein were moderately increased in the human papillomaviurs 11- and 59-infected tissues. PMID: 14748073
亚细胞定位：

Cytoplasm.
蛋白家族：

Cornifin (SPRR) family
组织特异性：

Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
数据库链接：

HGNC: 11262

OMIM: 182268

KEGG: hsa:6701

STRING: 9606.ENSP00000340703

UniGene: Hs.568239