Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人小而密低密度脂蛋白（sLDL）酶联免疫检测试剂盒（货号：CSB-E13350h），基于竞争法定量原理开发，适用于科研领域中对血清、血浆、唾液、尿液及组织匀浆样本中sLDL含量的精准检测。sLDL是低密度脂蛋白中颗粒更小、密度更高的亚型，其水平升高与动脉粥样硬化、冠心病等心血管疾病密切相关，是研究脂代谢异常的重要生物标志物。本试剂盒检测范围为0.312-20 nmol/mL，覆盖广泛的生物样本浓度需求，可灵敏识别样本中微量sLDL的存在。该试剂盒采用预包被板条和优化反应体系设计，操作流程标准化，支持科研人员高效开展心血管疾病发病机制研究、降脂药物作用效果评估、代谢综合征相关模型构建等实验。同时，其兼容唾液、尿液等非侵入性样本的特性，为纵向追踪研究或大规模样本分析提供了便利。产品严格遵循定量检测标准，实验结果稳定可靠，适用于体外实验环境下的基础研究、药理学研究及生物标志物探索等科研场景。
缩写：

sLDL
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, saliva, urine, tissue homogenates
检测范围：

0.312 nmol/mL-20 nmol/mL
灵敏度：

0.078 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	92-103
1:200	Average %	92
	Range %	86-96
1:400	Average %	104
	Range %	97-107
1:800	Average %	84
	Range %	80-88

回收率：

The recovery of human sLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-93
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.