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  4. Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

文献引用 (21) 说明书下载
  • 中文名称：
    人信号肽,CUB和EGF样结构域蛋白1(SCUBE1)酶联免疫试剂盒
  • 货号：
    CSB-E15005h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人信号肽、CUB和EGF样结构域蛋白1（SCUBE1）酶联免疫试剂盒（货号CSB-E15005h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的SCUBE1蛋白水平。SCUBE1是一种在细胞粘附、分化及信号转导中发挥作用的跨膜蛋白，其异常表达与心血管疾病、炎症反应及肿瘤微环境调控等研究领域密切相关。该试剂盒检测灵敏度高，线性检测范围覆盖0.625-40 ng/mL，通过预包被高亲和力捕获抗体和酶标检测抗体的协同作用，确保对目标蛋白的特异性识别与定量分析，操作流程兼容常规实验室设备。其优化的反应体系适用于基础科研中探索SCUBE1在疾病模型中的表达动态、信号通路关联性分析，或作为分子标志物在细胞实验、动物模型及临床前样本中的定量评估，为研究血管生成、组织修复及病理机制提供可靠工具。试剂盒包含标准品、预混试剂及操作说明书，支持用户快速开展重复性实验，满足分子生物学及转化医学研究的精准检测需求。
  • 别名：
    SCUB1_HUMAN ELISA Kit; SCUBE 1 ELISA Kit; SCUBE1 ELISA Kit; Signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit; Signal peptide, CUB domain, EGF like 1 ELISA Kit
  • 缩写：
    SCUBE1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %89
    Range %82-94
    1:10Average %96
    Range %91-105
    1:20Average %99
    Range %95-108
    1:40Average %94
    Range %86-102
  • 回收率：
    The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10699-110
    EDTA plasma (n=4)9284-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.670 2.592 2.631 2.502
    202.271 2.173 2.222 2.093
    101.631 1.615 1.623 1.494
    50.940 0.925 0.933 0.804
    2.50.512 0.502 0.507 0.378
    1.250.357 0.347 0.352 0.223
    0.6250.226 0.219 0.223 0.094
    00.130 0.127 0.129 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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SCUBE1 antibodies

SCUBE1 Proteins

靶点详情

  • 功能：
    Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
  • 基因功能参考文献：
    1. The results of this pioneering study indicate that SCUBE protein family appears to have a probable role in the pathogenesis and angiogenesis development in psoriasis and SCUBE 1 and 3 may be novel markers of angiogenesis in psoriasis. PMID: 28238185
    2. enhanced SCUBE1 concentrations are correlated with increasing severity and poor outcomes of aneurysmal subarachnoid hemorrhage patients, indicating SCUBE1 might have the potential to identify aneurysmal subarachnoid hemorrhage patients at risk of poor prognosis PMID: 27265525
    3. Increased serum SCUBE1 concentrations have close relation to increasing severity and poor prognosis of intracerebral hemorrhage PMID: 27496080
    4. SCUBE1 might be a novel prognostic biomarker after traumatic brain injury PMID: 26940951
    5. describe novel mechanisms in targeting S1 to the plasma membrane and demonstrate that N-glycans are required for S1 functions during primitive haematopoiesis in zebrafish. PMID: 26699903
    6. SCUBE1 may indicate hypercoagulability in patients with BC and thus help identify patients at greater risk of thrombosisSCUBE1 may indicate hypercoagulability in patients with breast cancer and thus help identify patients at greater risk of thrombosis PMID: 26438214
    7. SCUBE1 levels are a new biomarker that can be used in the differential diagnosis and monitoring of patients hospitalized with suspected CCHF. PMID: 23632301
    8. Primary prostatic cancer-associated fibroblasts (CAFs) were isolated and the expression of CAF markers, Dlk1 and SCUBE1 transcripts were characterised. PMID: 23136397
    9. SCUBE 1 levels in patients with gastric cancer were found higher compared to healthy subjects. PMID: 23220851
    10. SCUBE1 level, a potential acute ischemia marker, is elevated in hemodialysis patients with no clinical ischemic event. PMID: 22874483

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  • 亚细胞定位：
    Secreted. Cell membrane; Peripheral membrane protein.
  • 组织特异性：
    Detected in endothelial cells. Highly expressed in platelets. Stored in platelet alpha granules, and transferred to the cell surface upon activation and aggregation. A smaller form, probably produced by limited proteolysis, after being released from the s
  • 数据库链接：

    HGNC: 13441

    OMIM: 611746

    KEGG: hsa:80274

    STRING: 9606.ENSP00000354080

    UniGene: Hs.133995

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