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  4. Human Serotonin N-acetyltransferase(AANAT) ELISA kit

Human Serotonin N-acetyltransferase(AANAT) ELISA kit

说明书下载
  • 中文名称：
    人芳烷基胺N-乙酰转移酶(AANAT)酶联免疫试剂盒
  • 货号：
    CSB-EL001022HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人芳烷基胺N-乙酰转移酶（AANAT）ELISA试剂盒（货号：CSB-EL001022HU）是一款基于夹心法原理的定量检测试剂盒，适用于分析血清、血浆、组织匀浆或细胞裂解液中的AANAT蛋白水平，检测灵敏度范围为31.25 pg/mL至2000 pg/mL。AANAT是调控褪黑激素合成的关键限速酶，通过催化5-羟色胺的乙酰化参与昼夜节律调节，其表达异常与睡眠障碍、季节性情绪失调及神经内分泌疾病相关。CUSABIO试剂盒采用特异性抗体对靶标进行双位点捕获与检测，可在4.5小时内完成实验流程，提供高灵敏度和稳定性的定量结果。科研人员可将其应用于神经生物学、时间生物学或内分泌学领域的机制研究，例如探究不同光照周期下AANAT的节律性表达、评估褪黑激素通路相关疾病的动物模型或细胞模型中酶活性变化，以及筛选影响生物钟调控的小分子化合物。试剂盒包含预包被96孔板、标准品、检测抗体及显色底物，支持多样本类型的高通量分析，为揭示AANAT在生理病理过程中的作用提供可靠工具。
  • 别名：
    AA-NAT ELISA Kit; Aanat ELISA Kit; Aralkylamine N-acetyltransferase ELISA Kit; Arylalkylamine N acetyltransferase ELISA Kit; Serotonin acetylase ELISA Kit; Serotonin N-acetyltransferase ELISA Kit; SNAT ELISA Kit; SNAT_HUMAN ELISA Kit
  • 缩写：
    AANAT
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AANAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 105
    Range % 103-109
    1:2 Average % 96
    Range % 92-98
    1:4 Average % 91
    Range % 86-96
    1:8 Average % 93
    Range % 89-97
  • 回收率：
    The recovery of human AANAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 99-108
    EDTA plasma (n=4) 92 87-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.141 2.302 2.222 2.121
    1000 1.626 1.711 1.669 1.568
    500 1.248 1.358 1.303 1.202
    250 0.791 0.760 0.776 0.675
    125 0.531 0.503 0.517 0.416
    62.5 0.311 0.331 0.321 0.220
    31.25 0.190 0.198 0.194 0.093
    0 0.097 0.105 0.101 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

AANAT antibodies

AANAT Proteins

靶点详情

  • 功能：
    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
  • 基因功能参考文献：
    1. Aggregate genetic variation in circadian rhythm and melatonin pathways were significantly associated with the risk of prostate cancer in data combining GAME-ON and PLCO, after Bonferroni correction (ppathway < 0.00625). The two most significant genes were NPAS2 (pgene = 0.0062) and AANAT (pgene = 0.00078); the latter being significant after Bonferroni correction. PMID: 28699174
    2. Data show that arylalkymine N-acetyltransferase (AANAT) levels and melatonin synthesis change after transient receptor potential channel 4 (TRPV4 channel) stimulation in ciliary body epithelial cells. PMID: 28368307
    3. these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences. PMID: 27339900
    4. There is dysregulation of the AANAT/ASMT/melatonin --> melatonin receptor axis in cholangiocarcinoma, which inhibited melatonin secretion and subsequently enhanced CCA growth. PMID: 21778461
    5. The expression of AANAT in epithelial cells of striated ducts in human submandibular glands. PMID: 21437622
    6. The functional expression of human SNA protein was closely associated with the elevated synthesis of N-acetylserotonin and melatonin in transgenic rice plants. PMID: 20586889
    7. Identified 17 sequence changes in AANAT gene of patients with major depression. Show evidence of the association of genetic variability in the AANAT gene with susceptibility to major depression. PMID: 20459461
    8. There is a significant increase in AANAT allele positivity at the single nucleotide polymorphism (alanine 129--> threonine) at between patients with DSPS & controls. AA-NAT could be a susceptibility gene for DSPS. PMID: 12736803
    9. Data suggest that the -263G/C single nucleotide polymorphism of arylalkylamine-N-acetyl-transferase (AA-NAT) may be an important determinant of the late/short sleep pattern. PMID: 15332344
    10. Single nucleotide polymorphisms in the AANAT gene identified thus far cannot explain the observed interindividual differences for nocturnal melatonin profiles in the subjects investigated. PMID: 18569588
    11. AANAT polymorphisms were not associated with adolescent idiopathic scoliosis. PMID: 18794762
    12. Observational study of genotype prevalence and gene-disease association. (HuGE Navigator) PMID: 17503170

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Acetyltransferase family, AANAT subfamily
  • 组织特异性：
    Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
  • 数据库链接：

    HGNC: 19

    OMIM: 600950

    KEGG: hsa:15

    STRING: 9606.ENSP00000250615

    UniGene: Hs.431417

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |