Human Serine/threonine-protein kinase LMTK3(LMTK3) ELISA kit

产品详情

产品描述：

人丝氨酸/苏氨酸蛋白激酶LMTK3(LMTK3)酶联免疫试剂盒（CSB-EL013017HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LMTK3含量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研领域中对疾病机制探索、药物靶点验证或细胞信号通路研究，例如通过检测不同处理条件下细胞或动物模型样本中LMTK3的动态变化，分析其在肿瘤进展中的功能，或评估潜在治疗药物对该靶点的调控效应。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LMTK3 ELISA Kit; KIAA1883 ELISA Kit; TYKLM3 ELISA Kit; Serine/threonine-protein kinase LMTK3 ELISA Kit; EC 2.7.11.1 ELISA Kit; Lemur tyrosine kinase 3 ELISA Kit
缩写：

LMTK3
Uniprot No.：

Q96Q04
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.7 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LMTK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of human LMTK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	105	100-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription. Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons.
亚细胞定位：

Membrane; Single-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Golgi apparatus membrane. Note=Punctate pattern in cell projections.
蛋白家族：

Protein kinase superfamily, Tyr protein kinase family
数据库链接：

HGNC: 19295

UniGene: Hs.207426