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  4. Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit

Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit

说明书下载
  • 中文名称：
    人分泌磷蛋白2(SPP2/SPP24)酶联免疫试剂盒
  • 货号：
    CSB-EL022604HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人分泌磷蛋白2（SPP2/SPP24）酶联免疫检测试剂盒（货号：CSB-EL022604HU），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的SPP2/SPP24蛋白。该蛋白属于分泌型磷蛋白家族，在骨骼代谢调控、矿物质稳态维持等生理过程中发挥重要作用，其异常表达与骨发育异常及肾脏疾病等病理机制相关。本试剂盒检测范围为15.6-1000 ng/ml，灵敏度达15.6 ng/ml，可在4.5小时内完成实验流程，适用于体外研究SPP2/SPP24在骨骼生物学、钙磷代谢调节、组织矿化或相关疾病模型中的表达水平变化。预包被的高特异性抗体可精准识别天然与重组蛋白，配套标准品及优化缓冲体系确保数据可靠性，支持多样本类型的高通量分析，为探索该蛋白的分子功能及病理关联提供灵敏稳定的科研工具。
  • 别名：
    SPP2 ELISA Kit; SPP24 ELISA Kit; Secreted phosphoprotein 24 ELISA Kit; Spp-24 ELISA Kit; Secreted phosphoprotein 2 ELISA Kit
  • 缩写：
    SPP2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/ml-1000 ng/ml
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 98    
    Range % 96-102    
    1:10 Average % 107    
    Range % 103-114    
    1:20 Average % 94    
    Range % 90-98    
    1:40 Average % 88    
    Range % 86-95    
  • 回收率：
    The recovery of human SPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 105 99-108    
    EDTA plasma (n=4) 94 90-98    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    1000 2.188 2.233 2.211 2.028    
    500 1.732 1.824 1.778 1.595    
    250 1.211 1.261 1.236 1.053    
    125 0.698 0.757 0.728 0.545    
    62.5 0.523 0.522 0.523 0.340    
    31.2 0.345 0.358 0.352 0.169    
    15.6 0.252 0.260 0.256 0.073    
    0 0.176 0.189 0.183 　    
        .          
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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SPP2 Proteins

靶点详情

  • 功能：
    Could coordinate an aspect of bone turnover.
  • 基因功能参考文献：
    1. Spp24 can inhibit the growth of prostate cancer and its bone metastasis induced by BMP2; spp24 may have great potential to be a therapeutic agent in clinical situations PMID: 27793899
    2. the two mutations of SPP2 have dominant negative effects and cellular accumulation of Spp-24 might be particularly toxic to photoreceptors and/or retinal pigment epithelium. SPP2 has a new role in retinal degeneration. PMID: 26459573
    3. Report describes a C-terminal fragment within SPP24 that is distinct from the cystatin domain and which independently binds to BMP-2 and TGF-beta. This fragment inhibited BMP-2 activity in an ectopic bone forming assay. PMID: 25418420
    4. Spp24, or proteolytic products of Spp24, bind cytokines of the TGF-beta superfamily and also activate intracellular signaling pathways [review] PMID: 25339413
    5. Spp24 truncation products have effects on osteoblastic differentiation mediated by kinase pathways that are independent of exogenous BMP/TGF-beta cytokines. PMID: 25501958
    6. Results describe the localization of the human secreted phosphoprotein 24 gene, its structure and the start of transcription in liver. PMID: 15062857
    7. spp24 contains at least 2 single nucleotide polymorphisms. Given its mechanism of action and sequence variability, SPP24 may be an interesting candidate for future studies of the genetic regulation of bone mass. PMID: 19375587

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    SPP2 family
  • 组织特异性：
    Detected in liver and plasma.
  • 数据库链接：

    HGNC: 11256

    OMIM: 602637

    KEGG: hsa:6694

    STRING: 9606.ENSP00000168148

    UniGene: Hs.444488

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |