Human S100 calcium-binding protein A8/A9 complex(S100A8/A9)Elisa kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO针对人S100钙结合蛋白A8/A9复合物（S100A8/A9）的定量检测试剂盒，采用双抗体夹心酶联免疫吸附法（ELISA）原理，适用于血清、血浆及组织匀浆样本的科研分析。S100A8/A9是由S100A8和S100A9亚基组成的异源二聚体，属于钙结合蛋白家族成员，在炎症反应、免疫调节及细胞信号传导中发挥重要作用，其异常表达与自身免疫性疾病、感染性疾病及肿瘤微环境调控等研究领域密切相关。试剂盒检测灵敏度高，线性范围为1.56-100 ng/mL，可精准检测低丰度样本，实验操作流程包含标准品预稀释及约4.5小时孵育步骤。该产品适用于体外研究，如炎症机制探索、疾病模型构建、药物干预效果评估或生物标志物相关性分析，为研究感染性病变、肿瘤进展、代谢综合征等病理过程中S100A8/A9的动态变化提供可靠工具。试剂盒包含预包被特异性抗体的96孔板、标准品、检测抗体及显色底物等完整组分，支持科研人员高效完成样本检测与数据分析。
别名：

N/A
缩写：

S100A8/A9
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A8/A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-92
1:2	Average %	95
	Range %	91-98
1:4	Average %	92
	Range %	87-98
1:8	Average %	95
	Range %	90-103

回收率：

The recovery of human S100A8/A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	92	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.