Human RING finger protein 207(RNF207) ELISA kit

Human RING finger protein 207(RNF207) ELISA kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

人环指蛋白207(RNF207)酶联免疫试剂盒
货号：

CSB-EL019870HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

人环指蛋白207(RNF207)酶联免疫试剂盒（CSB-EL019870HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RNF207含量。RNF207是一种E3泛素连接酶，参与蛋白质泛素化过程，与心肌病和心脏能量代谢相关。研究发现，RNF207在心肌细胞中表达，可能通过影响HERG蛋白的转运和定位来调节动作电位持续时间，从而影响心脏功能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒检测灵敏度为15.6 pg/mL，线性检测范围覆盖15.6-1000 pg/mL，可精准测定低丰度样本中RNF207的表达量，适用于探索其在心肌细胞代谢、组织损伤修复或病理状态下的分子机制研究。提供标准品、检测抗体及全套优化缓冲液，支持科研人员对心血管疾病模型、基因编辑细胞系或药物干预实验中的RNF207动态变化进行定量分析，为功能研究与分子互作机制探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

RNF207 ELISA Kit; C1orf188 ELISA Kit; RING finger protein 207 ELISA Kit
缩写：

RNF207
Uniprot No.：

Q6ZRF8
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human RNF207 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	86
	Range %	81-90
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human RNF207 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	101	95-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.719	2.519	2.619	2.500
500	2.172	2.072	2.122	2.003
250	1.504	1.607	1.556	1.437
125	0.996	1.061	1.029	0.910
62.5	0.653	0.623	0.638	0.519
31.2	0.362	0.380	0.371	0.252
15.6	0.252	0.261	0.257	0.138
0	0.123	0.115	0.119

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Plays a role in cardiac repolarization possibly by stabilizing membrane expression of the potassium channel KCNH2/HERG, or by assisting its synthesis, folding or export from the endoplasmic reticulum, in a heat shock protein-dependent manner.
基因功能参考文献：
1. Data indicate that plasma E3 Ligase Rnf207 (ring finger protein 207) level in acute myocardial infarction patients increased significantly compared with that in healthy people. PMID: 25599194
2. RNF207 is an important regulator of action potential duration, likely via effects on HERG trafficking and localization in a heat shock protein-dependent manner. PMID: 25281747
3. Meta-analysis and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19305408
4. Genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19305409
亚细胞定位：

Cytoplasm.
数据库链接：

HGNC: 32947

OMIM: 616923

KEGG: hsa:388591

STRING: 9606.ENSP00000367173

UniGene: Hs.716549

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.