Human Protein notum homolog(NOTUM) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人notum蛋白同源物(NOTUM)酶联免疫试剂盒（CSB-EL015955HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOTUM含量。NOTUM作为一个靶点，它是羧基酯酶家族成员。在研究机制上，其可通过去除Wnt蛋白的棕榈酰基抑制Wnt信号通路。在组织发育、干细胞维持等生理过程以及肿瘤、神经退行性疾病等病理进程中发挥作用，是潜在治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，在组织再生、脂肪代谢及神经退行性疾病研究中具有重要科研价值。适用于探究NOTUM在代谢性疾病、肿瘤微环境调控或神经发育相关机制中的表达变化，为体外实验研究提供可靠的蛋白定量支持，满足基础科研中对Wnt通路相关分子调控机制的研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

NOTUM ELISA Kit; notum pectinacetylesterase homolog (Drosophila) ELISA Kit; Notum pectinacetylesterase homolog ELISA Kit; NOTUM_HUMAN ELISA Kit; Protein notum homolog ELISA Kit
缩写：

NOTUM
Uniprot No.：

Q6P988
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	85
	Range %	80-90

回收率：

The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	106	100-114

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
基因功能参考文献：
1. NOTUM expression was increased in metastatic cells. Proliferation was suppressed by inhibiting expression of NOTUM. Knockdown of NOTUM genes inhibited proliferation as well as migration, with possible involvement of p38 and c-JUN N-terminal kinase in this process. PMID: 30343282
2. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
3. Kinetic and mass spectrometric analyses of human proteins show that Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins and thus constitutes the first known extracellular protein deacylase PMID: 25731175
4. Overexpression of NOTUM is associated with hepatocellular carcinoma PMID: 18429952
亚细胞定位：

Secreted.
蛋白家族：

Pectinacetylesterase family, Notum subfamily
组织特异性：

Rarely expressed in adult normal tissues.
数据库链接：

HGNC: 27106

OMIM: 609847

KEGG: hsa:147111

STRING: 9606.ENSP00000387310

UniGene: Hs.106137