Human Protein dopey-2(DOPEY2) ELISA kit

Human Protein dopey-2(DOPEY2) ELISA kit

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中文名称：

人dopey-2蛋白(DOPEY2)酶联免疫试剂盒
货号：

CSB-EL007119HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人dopey-2蛋白(DOPEY2)酶联免疫试剂盒（CSB-EL007119HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DOPEY2含量。DOPEY2是一种蛋白质编码基因，与认知和胚胎模式指定有关，位于早期内体膜上。其可能参与调节货物蛋白的膜运输，与ATP9A和MON2一起调节SNX3逆反器介导的内体排序，避免WLS被溶酶体降解。DOPEY2的研究涉及其在细胞糖基化和囊泡运输中的影响，以及其在胚胎神经发生中与Pcdh7协同调控神经干细胞增殖和分化的作用。试剂盒检测范围为32 pg/mL-1200 pg/mL，支持科研工作者在神经生物学、细胞代谢调控或疾病机制研究中开展定量分析；适用于脑组织、外周血样本或体外培养细胞模型中DOPEY2表达水平的高通量检测，为探究其在神经退行性疾病、代谢综合征等病理过程中的作用提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

DOP1B ELISA Kit; C21orf5 ELISA Kit; DOPEY2 ELISA Kit; KIAA0933Protein dopey-2 ELISA Kit
缩写：

DOPEY2
Uniprot No.：

Q9Y3R5
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

32 pg/mL-1200 pg/mL
灵敏度：

8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DOPEY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	89
	Range %	83-95

回收率：

The recovery of human DOPEY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	102-106
EDTA plasma (n=4)	93	90-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.313	2.451	2.382	2.335
640	1.754	1.802	1.778	1.731
320	1.012	1.032	1.022	0.975
80	0.345	0.355	0.350	0.303
32	0.173	0.174	0.174	0.127
0	0.045	0.048	0.047

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

May play a role in regulating membrane trafficking of cargo proteins. Together with ATP9A and MON2, regulates SNX3 retromer-mediated endosomal sorting of WLS away from lysosomal degradation.
基因功能参考文献：
1. The differentially expressed C21orf5 gene in the medial temporal-lobe system could play a role in Down syndrome. PMID: 12767918
2. C21orf5 selective expression in the key brain structures for learning and memory suggests that C21orf5 overexpression could participate in mental retardation pathogenesis in Down syndrome patients. PMID: 16276086
3. C21orf5, could play a role in brain morphogenesis and, when overexpressed, it could participate in neurological features and mental retardation observed in DS patients. PMID: 16303751
4. Results quantify and statistically analyze, for the first time, DOPEY2 expression variations in different regions of the Down syndrome human fetal brains and to compare them to corresponding normal brains. PMID: 19460634
亚细胞定位：

Early endosome membrane. Golgi apparatus membrane; Peripheral membrane protein.
蛋白家族：

Dopey family
组织特异性：

Ubiquitously expressed. Overexpressed in lymphoblasts from Down syndrome patients.
数据库链接：

HGNC: 1291

OMIM: 604803

KEGG: hsa:9980

STRING: 9606.ENSP00000382104

UniGene: Hs.204575

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.