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  4. Human Protein S100-A7A(S100A7A) ELISA kit

Human Protein S100-A7A(S100A7A) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人S100-A7A蛋白(S100A7A)酶联免疫试剂盒
  • 货号：
    CSB-EL020636HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人S100-A7A蛋白（S100A7A）酶联免疫检测试剂盒（货号：CSB-EL020636HU），基于双抗体夹心法定量检测样本中S100A7A蛋白的浓度。S100A7A属于钙结合蛋白S100家族成员，参与皮肤炎症反应调控和细胞增殖分化，其异常表达与银屑病、肿瘤微环境重塑等病理过程相关。试剂盒可检测血清、血浆、组织匀浆及尿液样本，检测灵敏度下限为0.312 ng/mL，线性范围覆盖至20 ng/mL，适用于体外定量分析低丰度靶蛋白的表达水平。产品预包被高特异性捕获抗体，搭配生物素标记的检测抗体及链霉亲和素-HRP系统，通过显色反应实现目标蛋白的精准定量。该试剂盒可广泛用于基础医学研究中S100A7A蛋白的功能探索，例如皮肤疾病模型中的炎症标志物检测、肿瘤组织微环境相关蛋白表达分析，或通过体液样本评估生物分子动态变化，为分子机制研究提供灵敏可靠的工具。实验操作兼容常规酶标仪检测，支持多样本类型灵活选择，满足科研场景中复杂样本的检测需求。
  • 别名：
    S100A7A ELISA Kit; S100A15 ELISA Kit; S100A7L1 ELISA Kit; Protein S100-A7A ELISA Kit; S100 calcium-binding protein A15 ELISA Kit; S100 calcium-binding protein A7-like 1 ELISA Kit; S100 calcium-binding protein A7A ELISA Kit
  • 缩写：
    S100A7A
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, urine
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human S100A7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 92
    Range % 89-96
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 91
    Range % 82-97
  • 回收率：
    The recovery of human S100A7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 93-101
    EDTA plasma (n=4) 86 81-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.834 2.912 2.873 2.758
    10 2.175 2.017 2.096 1.981
    5 1.286 1.218 1.252 1.137
    2.5 0.654 0.664 0.659 0.544
    1.25 0.397 0.386 0.392 0.277
    0.625 0.249 0.242 0.246 0.131
    0.312 0.167 0.162 0.165 0.050
    0 0.115 0.114 0.115 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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S100A7A Proteins

靶点详情

  • 功能：
    May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases.
  • 基因功能参考文献：
    1. S100A15 retains the ability to coordinate zinc through incorporation of an exogenous ligand resulting in a unique zinc-binding site among S100 proteins. PMID: 22747601
    2. Molecular cloning and characterization of alternatively spliced S100A15 isoforms from psoriatic skin. PMID: 12923069
    3. The S100A15 protein has been expressed & purified to homogeneity in 2 crystal forms. Crystals of form I are triclinic. Crystals of form II are monoclinic & have a solvent content of 51.8%, corresponding to the presence of 1 monomer in the asymmetric unit. PMID: 16682778
    4. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin PMID: 17620096
    5. E. coli modulates S100A15 and IL-8 expression of keratinocytes by recognition through TLR4. PMID: 17625598
    6. study defines RAGE (receptor for advanced glycation end products) as the hS100A7 receptor, whereas hS100A15 functions through a Gi protein-coupled receptor PMID: 18606705

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Overexpressed in psoriasis.
  • 数据库链接：

    HGNC: 21657

    OMIM: 617427

    KEGG: hsa:338324

    UniGene: Hs.442337

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |