Human Protein FAM3C(FAM3C) ELISA kit

Human Protein FAM3C(FAM3C) ELISA kit

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中文名称：

人FAM3C蛋白(FAM3C)酶联免疫试剂盒
货号：

CSB-EL008228HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人FAM3C蛋白(FAM3C)酶联免疫试剂盒（CSB-EL008228HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAM3C含量。FAM3C，也称为ILEI，是FAM3家族的一员，主要存在于分泌性上皮细胞中。它在视网膜层状形成、上皮间质转化和胰腺癌发生中发挥重要作用。研究机制上，FAM3C可能通过调节细胞分化和组织重塑影响细胞行为，其具体作用机制尚在研究中。试剂盒检测范围为1.56 ng/ml- 100 ng/ml，适用于体外培养细胞模型、动物实验组织样本或人类体液样本中FAM3C蛋白表达水平的动态监测，为探索其在肿瘤微环境调控、代谢性疾病机制或药物干预研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Fam3c ELISA Kit; FAM3C_HUMAN ELISA Kit; Family with sequence similarity 3 member C ELISA Kit; GS3786 ELISA Kit; ILEI ELISA Kit; Interleukin-like EMT inducer ELISA Kit; Predicted osteoblast protein ELISA Kit; Protein FAM3C ELISA Kit
缩写：

FAM3C
Uniprot No.：

Q92520
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/ml - 100 ng/ml
灵敏度：

0.39 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM3C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	96
	Range %	92-101
1:4	Average %	92
	Range %	87-98
1:8	Average %	97
	Range %	94-101

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

100

1.941

2.010

1.976

1.875

1.476

1.570

1.523

1.422

1.073

1.061

1.067

0.966

12.5

0.702

0.751

0.727

0.626

6.25

0.404

0.432

0.418

0.317

3.12

0.312

0.322

0.317

0.216

1.56

0.201

0.205

0.203

0.102

0.099

0.101

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.
基因功能参考文献：
1. The ILEI appears to play a crucial role in mediating TGF-beta1-induced EMT through the Akt and ERK pathways, which may provide a therapeutic target for the treatment of fibrotic kidney diseases. PMID: 29336056
2. Covalent dimerization of ILEI is associated with breast cancer metastasis. PMID: 28837266
3. Data suggest that ILEI does not form interleukin-like four helix-bundle structures; ILEI exists as monomers and as covalent dimers; ILEI dimer exhibits a trans-linked domain swap converting an intramolecular disulfide to an intermolecular disulfide; dimeric ILEI appears to be involved in neoplastic invasiveness. PMID: 28751379
4. Results demonstrate that phenotype switching regulates ILEI expression, and that ILEI regulates partial phenotype switching in MITF-low melanoma cell lines. PMID: 28545079
5. Ectopic overexpression of UBE4A, but not UBE3C, in cells was downregulated in vitro migration and invasion in these cells. Cumulatively, our data reveals a novel post-translational regulatory mechanism of regulating ILEI1 expression, a protein required for metastatic progression in prostate cancer cells PMID: 27862841
6. Study used immunohistochemical staining and biochemical fractionation to examine the regional distribution and subcellular localization of ILEI in mouse brains and in autopsy brains of patients with Alzheimer's disease; results suggest that a decline of ILEI expression may cause accumulation of Abeta in the brain and the eventual development of Alzheimer's disease. PMID: 27256505
7. FAM3C expression was dramatically increased in esophageal squamous cell carcinoma and might serve as a valuable prognostic indicator for esophageal squamous cell carcinoma patients after surgery PMID: 26498278
8. Altered subcellular ILEI localization strongly correlates with high tumor cell-associated uPAR protein expression. PMID: 25212966
9. Our findings indicate that cytoplasmic ILEI expression is a potential marker of EMT and tumour progression in colorectal cancer. ILEI is an independent predictive factor associated with poor prognosis in colorectal cancer. PMID: 24738665
10. ILEI could induce, as well as maintain, CD24(low)CD44(high) subpopulation in A549 cells treated with TGF-beta, which might explain its capability to induce metastatic progression. PMID: 24072492
11. At the FAM3C gene locus where rs7776725 was located, we identified several other SNPs (rs4727922, rs1803389, rs718766 and rs7793554) that were also associated with BMD. PMID: 22159821
12. A cytokine-like protein found in almost all tissues. PMID: 12160727
13. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors. PMID: 16959614
14. Data show that FAM3C is decreased in the CSF of temporal lobe epilepsy patients. PMID: 19109932
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亚细胞定位：

Secreted. Cytoplasmic vesicle. Note=Cytoplasmic in some cancer cells.
蛋白家族：

FAM3 family
组织特异性：

Present in most secretory epithelia (at protein level).
数据库链接：

HGNC: 18664

OMIM: 608618

KEGG: hsa:10447

STRING: 9606.ENSP00000353025

UniGene: Hs.272375

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.