Human Prostaglandin F2α,PGF2α ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人前列腺素F2α(PGF2α)酶联免疫试剂盒（CSB-E10142h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PGF2α含量。PGF2α 是重要靶点。它作为前列腺素家族一员，在人体生理过程如生殖、心血管调节等方面发挥作用。研究机制上，其通过与特定受体结合，激活下游信号通路，影响细胞功能和生理反应，在相关疾病治疗领域具潜在研究价值。试剂盒检测范围为1.56 pg/mL-100 pg/mL，该产品适用于探索PGF2α在生殖系统模型（如前列腺功能研究）、心血管疾病机制、炎症反应调控及药物干预实验中的生物学作用，为细胞信号通路研究、疾病分子机制解析及潜在生物标志物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

PGF2 alpha,Prostaglandin F2 alpha
缩写：

PGF2α
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	102
	Range %	97-107
1:4	Average %	93
	Range %	85-99
1:8	Average %	98
	Range %	91-104

回收率：

The recovery of human PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	88	81-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.