Human Prostaglandin D2(PGD2) ELISA kit

Human Prostaglandin D2(PGD2) ELISA kit

产品详情

引用文献

产品评价

相关问答

产品详情

引用文献

产品评价

相关产品

相关问答

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

人前列腺素D2(PGD2)酶联免疫试剂盒
货号：

CSB-E13898h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
相关问答

产品描述：

人前列腺素D2(PGD2)酶联免疫试剂盒（CSB-E13898h）为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、组织匀浆、尿液样本中的PGD2含量。PGD2即前列腺素D2，是一种重要的脂质介质。它参与多种生理病理过程，如炎症反应、睡眠调节等。在研究机制上，PGD2通过与特定受体结合，激活下游信号通路，调控细胞的功能和基因表达，是炎症相关疾病研究的热门靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品可广泛应用于炎症性疾病机制研究、药物筛选模型中的PGD2动态监测、泌尿系统相关科研中的生物标志物分析，以及代谢组学研究中的脂质介质定量检测，为体外实验和动物模型研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

PGD2
种属：

Homo sapiens (Human)
样本类型：

serum, cell culture supernates, tissue homogenates, urine
检测范围：

23.5 pg/mL-1500 pg/mL
灵敏度：

5.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-94
1:2	Average %	98
	Range %	91-105
1:4	Average %	99
	Range %	94-110
1:8	Average %	94
	Range %	87-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.985	1.854	1.920	1.840
750	1.403	1.323	1.363	1.283
375	0.912	0.886	0.899	0.819
187.5	0.545	0.525	0.535	0.455
94	0.371	0.359	0.365	0.285
47	0.204	0.198	0.201	0.121
23.5	0.129	0.126	0.128	0.048
0	0.080	0.079	0.080

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Calcitonin gene-related peptide promotes epithelial reparative and anti-colitic functions of IL-4 educated human macrophages BE Callejas, JA Sousa, KL Flannigan,American Journal of Physiology-Gastrointestinal and Liver Physiology,2024
Exploring Long Non-Coding RNAs Associated with IP3/DAG Signaling Pathway as Potential Biomarkers Involved in Mast Cell Degranulation in Chronic Spontaneous Urticaria with 2-Year Follow-Up Y Liang,Journal of Inflammation Research,2022
Cyclooxygenase 1 (COX1) as an indicator of sperm quality in humans Y Liu,Andrologia,2020

■ 常见问题解答

We have previously ordered these kits from you and have been running the project since 2014. I want to keep the same assays as before but I am wondering if you can provide any quality control material? In the past we didn’t have quality control material available. I understand that the kit comes with the required standards but it would be great if a positive and negative control could be purchased to be run with the kit as well. I would purchase these separately if available.Any recommendations as controls for this kit?

Thanks for your inquiry.
CSB-E13898h
The standards in the kit can be used for quantitative analysis and control, so we didn't provide extra control.
Our kit is for research use only and has been optimized. Customers only need to do blank control during the assay and additional quality control is not needed. Pls let me know if you have any further questions. Thank you.

显示更多

收起更多

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.