Human Proadrenomedullin (Pro-ADM) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人肾上腺髓质素前体（Pro-ADM）是一种由肾上腺髓质分泌的多肽前体蛋白，经酶切后可生成具有生物活性的肾上腺髓质素（ADM），参与调节心血管功能、炎症反应及体液平衡等生理过程，其表达水平与心血管疾病、败血症等病理状态密切相关，是潜在的重要生物标志物。本试剂盒（货号：CSB-E14356h）采用双抗体夹心法原理，可定量检测人血清、血浆、细胞培养上清、尿液及组织匀浆中的Pro-ADM蛋白，检测灵敏度为0.312 pmol/L，线性范围为0.312-20 pmol/L，适用于低丰度样本的精准分析。该产品支持多种生物样本类型，能够满足基础医学研究中疾病机制探索、药物作用评估或生物标志物筛选等需求，例如通过检测循环系统中Pro-ADM的动态变化，为心血管疾病或全身性炎症相关研究提供数据支持。本产品仅限科学研究使用，操作便捷且重复性良好，配套提供标准化实验方案与数据分析指南，助力科研人员高效开展实验。
缩写：

Pro-ADM
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：

0.312 pmol/L-20 pmol/L
灵敏度：

0.078 pmol/L
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	85
	Range %	80-92
1:400	Average %	95
	Range %	90-100
1:800	Average %	99
	Range %	92-110
1:1600	Average %	92
	Range %	82-105

回收率：

The recovery of human Pro-ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-100
EDTA plasma (n=4)	101	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.