Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit

产品详情

产品描述：

本试剂盒（货号：CSB-E05149h）专为科研领域设计，采用双抗体夹心法原理对人前心钠肽（Pro-ANP）进行定量检测，适用于血清、血浆及组织匀浆样本的分析。Pro-ANP是心钠肽激素的前体蛋白，主要由心肌细胞分泌，参与调节血压和体液平衡，其浓度变化与心脏功能状态密切相关，是心血管疾病研究的重要生物标志物。试剂盒检测灵敏度优异，线性范围为62.5-4000 pg/mL，可精准覆盖生理及病理条件下Pro-ANP的表达水平。实验操作基于酶标板预包被的特异性捕获抗体，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配合标准品梯度建立标准曲线完成定量分析。该产品广泛应用于心血管疾病机制研究、药物干预效果评估、动物模型心脏功能分析等科研场景，尤其适用于探索心力衰竭、高血压及相关代谢性疾病中Pro-ANP的动态变化规律，为实验室提供稳定可靠的检测工具。
缩写：

Pro-ANP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	85
	Range %	80-89
1:4	Average %	92
	Range %	87-98
1:8	Average %	104
	Range %	96-108

回收率：

The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	87	82-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.