Human Platelet factor 4 variant(PF4V1) ELISA kit

Human Platelet factor 4 variant(PF4V1) ELISA kit

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中文名称：

人血小板因子4变体(PF4V1)酶联免疫试剂盒
货号：

CSB-EL017810HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人血小板因子4变体(PF4V1)酶联免疫试剂盒（CSB-EL017810HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PF4V1含量。PF4V1，也称为CXCL4L1，是一种血小板相关趋化因子，具有抗血管生成功能，与炎症、血管生成和癌症有关。其研究机制涉及结构差异和功能特性，如与肝素结合能力较弱，以及作为血管生成抑制剂和内皮细胞趋化性抑制剂的作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于体外科研场景，如探索PF4V1在疾病模型中的表达调控机制、评估药物或基因干预对血小板活化的影响，或分析不同组织来源样本中该因子的动态变化，为细胞信号通路研究及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

PF4V1 ELISA Kit; CXCL4V1 ELISA Kit; SCYB4V1 ELISA Kit; Platelet factor 4 variant ELISA Kit; C-X-C motif chemokine 4 variant ELISA Kit; CXCL4L1 ELISA Kit; PF4alt ELISA Kit; PF4var1) [Cleaved into: Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)] ELISA Kit
缩写：

PF4V1
Uniprot No.：

P10720
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PF4V1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-99
1:2	Average %	98
	Range %	95-103
1:4	Average %	102
	Range %	94-106
1:8	Average %	95
	Range %	90-98

回收率：

The recovery of human PF4V1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	99	96-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.945	3.094	3.020	2.831
20	2.266	2.399	2.333	2.144
10	1.532	1.497	1.515	1.326
5	0.775	0.776	0.776	0.587
2.5	0.453	0.454	0.454	0.265
1.25	0.369	0.341	0.355	0.166
0.625	0.230	0.250	0.240	0.051
0	0.184	0.194	0.189

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

CXCL4L1 May Help Differentiate Benign from Malignant Pulmonary Lesions and Predicts Prognosis of Patients with Lung Cancer L Zhang,Cancer management and research,2022
Downregulation of serum CXCL4L1 predicts progression and poor prognosis in prostate cancer patients treated by radical prostatectomy Zhang M, et al,Asian journal of andrology,2019
Heparin-mimicking polyethersulfone Membranes-hemocompatibility, cytocompatibility, antifouling and antibacterial properties Shuang-Si Li. et al,Journal of Membrane Science,2016
Nanofibrous Heparin and Heparin-mimicking Multilayers as Highly Effective Endothelialization and Antithrombogenic Coatings Nie C et al,Biomacromolecules,2015

功能：

Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro).
基因功能参考文献：
1. Fibstatin and CXCL4L1 cooperate to inhibit endothelial cell proliferation, migration and tubulogenesis in vitro. PMID: 23747987
2. fibstatin and CXCL4L1 have cooperative roles in inhibition of tumor invasion in lymph nodes PMID: 23747987
3. Structural insight into the novel functional properties of human CXCL4L1. PMID: 23536183
4. PF-4var/CXCL4L1 predicts outcome in stable coronary artery disease patients with preserved left ventricular function PMID: 22384011
5. both CXCR3A and CXCR3B are implicated in the chemotactic and vascular effects of CXCL4L1. PMID: 20980681
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
数据库链接：

HGNC: 8862

OMIM: 173461

KEGG: hsa:5197

STRING: 9606.ENSP00000226524

UniGene: Hs.72933

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.