Human Pepsinogen I ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人胃蛋白酶原I酶联免疫试剂盒（CSB-E17538h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PG I含量。PG I（胃蛋白酶原I）是胃中的一种消化酶，其生物学功能包括消化食物中的蛋白质。研究显示，PG I在心血管系统中具有重要作用，其合成能力由内向外依次减弱，以内皮细胞合成能力最强。PG I的研究机制涉及其对心血管系统的影响，以及其在胃癌诊断中的应用。试剂盒检测范围为6.25 ng/mL-400 ng/mL，支持科研人员高效开展胃功能研究、胃黏膜损伤机制探索或药物干预效果评估等实验。其宽泛的线性范围和多类型样本兼容性，特别适用于纵向研究或不同实验条件间的数据比对，为胃相关疾病的分子机制解析及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PG I
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	106
	Range %	100-115
1:4	Average %	97
	Range %	94-112
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.