Human Pepsin,PG ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人胃蛋白酶（PG）是一种由胃黏膜主细胞分泌的蛋白水解酶，在胃液酸化和蛋白质消化过程中发挥重要作用，其含量变化与胃黏膜健康状态密切相关。本试剂盒（货号：CSB-E08919h）采用双抗体夹心法定量检测人血清、血浆、尿液及组织匀浆样本中的PG浓度，检测灵敏度达31.25 pg/mL，线性范围覆盖31.25-2000 pg/mL，实验全程约需4.5小时。试剂盒包含预包被捕获抗体的96孔板、检测抗体及标准品，通过酶标仪在450 nm波长下测定显色强度实现定量分析，适用于消化系统疾病机制研究、胃功能相关生物标志物探索、营养代谢评估等科研领域。样本处理建议离心去除颗粒物，组织样本需按1:9比例制备匀浆后检测。该产品经特异性验证，与人胃蛋白酶原I/II及其他常见蛋白无交叉反应，实验稳定性良好，可为胃蛋白酶表达水平研究提供可靠工具。
别名：

PGA3 ELISA Kit; Pepsin A-3 ELISA Kit; EC 3.4.23.1 ELISA Kit; Pepsinogen-3 ELISA Kit
缩写：

PG
Uniprot No.：

P0DJD8
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	84-95
1:200	Average %	93
	Range %	88-99
1:400	Average %	99
	Range %	95-104
1:800	Average %	101
	Range %	96-108

回收率：

The recovery of human PG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-104
EDTA plasma (n=4)	94	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
基因功能参考文献：
1. Low Pepsinogen I is associated with precancerous gastric lesions. PMID: 25292040
2. High serum Pepsinogen I level is associ9ated with the progression of gastric precancerous lesions. PMID: 24895149
3. Significantly higher AUC was observed for the PGI/PGII ratio and is associated with atrophy and intestinal metaplasia. PMID: 23886209
4. A significant negative correlation was found between the degree of corpus atrophy and the level of serum pepsinogen-I in atrophic gastritis patients with gastroesophageal reflux. PMID: 21175799
5. Higher levels of Pepsinogen I is associated with early gastric cancer PMID: 15688378
6. Helicobacter pylori eradication improves gastric histology and decreases serum gastrin, pepsinogen I and pepsinogen II levels in patients with duodenal ulcer. PMID: 17559360
7. pepsinogens may have a role in esophageal squamous dysplasia PMID: 18844222
8. Barrett's esophagus is characterized by the absence of Helicobacter pylori infection and high levels of serum pepsinogen I concentration in Japan. PMID: 19196398
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亚细胞定位：

Secreted.
蛋白家族：

Peptidase A1 family
数据库链接：

HGNC: 8885

OMIM: 169700

KEGG: hsa:643834

STRING: 9606.ENSP00000322192

UniGene: Hs.601055