Human Pancreastatin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人胰抑制素（Pancreastatin）是一种由胰腺分泌的调节性多肽，可通过抑制胰岛素分泌和促进胰高血糖素释放参与糖代谢调控，在能量平衡及代谢性疾病研究中具有重要价值。本试剂盒（货号：CSB-E09209h）基于双抗体夹心法定量检测人血清、血浆或组织匀浆中的Pancreastatin含量，检测灵敏度达31.25 pg/mL，线性范围覆盖31.25-2000 pg/mL，适用于微量样本的精准分析。该试剂盒采用高特异性配对抗体，确保检测结果的特异性和重复性，操作流程包含预包被板、生物素化检测抗体及链霉亲和素-HRP系统，可在4.5小时内完成检测。科研人员可将其应用于探究Pancreastatin在糖尿病、肥胖症等代谢异常病理机制中的作用，或用于评估药物干预、基因编辑等实验模型中该蛋白的动态表达水平变化，为相关基础研究提供可靠工具。
缩写：

Pancreastatin
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pancreastatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-110
1:2	Average %	97
	Range %	91-105
1:4	Average %	87
	Range %	82-93
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human pancreastatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	90	85-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.