Human Orexin B,OX-B ELISA Kit

产品详情

产品描述：

人食欲素/阿立新B(OX-B)酶联免疫试剂盒（CSB-E09863h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OX-B含量。人食欲素/阿立新B（OX-B）是一种由下丘脑神经元分泌的神经肽，能促进食欲、调节睡眠和觉醒。研究发现，OX-B与食欲素受体结合后，可调节摄食行为、睡眠周期及情绪等生理过程。针对OX-B及其受体的研究，为治疗肥胖、失眠等疾病提供了新的治疗靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于神经生物学研究中食欲素系统的功能解析、代谢调节机制探索，或睡眠障碍动物模型中的生物标志物分析，为中枢神经系统调控研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

OX-B
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	95-111
1:2	Average %	100
	Range %	93-104
1:4	Average %	94
	Range %	86-97
1:8	Average %	100
	Range %	92-104

回收率：

The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	108	97-112

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.